| Project/Area Number |
15K14858
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Miyake Masami 大阪府立大学, 生命環境科学研究科, 教授 (10251175)
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| Co-Investigator(Kenkyū-buntansha) |
安木 真世 大阪府立大学, 生命環境科学研究科, 准教授 (40589008)
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| Research Collaborator |
Sarker Mahfuzur R.
SAKANOUE Hideyo
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細胞接着 / レセプター / 定着因子 / 発芽 / エンテロトキシン / 芽胞 / ウェルシュ菌 / 消化管定着 |
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| Outline of Final Research Achievements |
We have tried to evaluate the adherence of Clostridium perfringens to human intestinal epithelial cells. In this study, we established an in vitro adherence assay and examined the adherence of spores of C. perfringens to human intestinal Caco-2 cells. Adherences of spores both of strain SM101 (a derivative of FBI strain NCTC8798) and of strain NCTC8239 (FBI strain) were observed within 15 minutes, and reached plateau at 60 min after inoculation. Scanning Electron microscopy and transmission electron microscopy revealed tight association of spores on the surface of Caco-2 cells. On the other hand, the adherence of vegetative cells both of the strains could not be confirmed by the same procedures. The results suggest that C. perfringens spores adhere to intestinal epithelial cells in vivo, although its biological significance remains to be determined.
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