Usefulness analysis of nerve growth factor in horse stress-related diseases as an etiology, the diagnosis parameter

• Project/Area Number: 15K14868
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Matsuda Hiroshi 東京農工大学, (連合)農学研究科(研究院), 教授 (80145820)
• Research Collaborator: ISHIZAKA Saori ; SATO Hiroaki ; TOISHI Yuko
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経成長因子 / ウマ / ストレス / 診断マーカー / サイトカイン / 輸送ストレス / クローニング / 相同性 / 測定系

Outline of Final Research Achievements

In this study, I focused on nerve growth factor (NGF) to clarify the etiology and diagnostic significance in horse diseases secondary to the transportation stress. In addition, I attempted to establish an assay system for horse NGF as the biomarker. The results are as follows: 1) the sequence of horse NGF was identified and homology was more than 90% in comparison to the other animals. 2) an ELISA system for horse NGF was developed with a commercial antibody. 3) NGF levels were increased in peripheral blood of transportation-stressed horses using the established ELISA. Thus, I established a NGF assay system and usefulness of the NGF in the horse was confirmed.

Research Products

• [Int'l Joint Research] ウィーン獣医科大学(オーストリア)
• [Int'l Joint Research] ウイーン獣医科大学(オーストリア)
• [Journal Article] Cloning and expression of equine beta-nerve growth factor. (2016)
  • Author(s): Amagai Y, Sato H, Ishizaka S, Matsuda K, Aurich C, Tanaka A, Matsuda H
  • Journal Title: J Equine Vet Sci Volume: 45 Pages: 28-31
  • DOI: 10.1016/j.jevs.2016.06.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Changes in serum NGF levels after the exercise load in dogs: a pilot study (2016)
  • Author(s): Ando I, Karasawa K, Matsuda H, Tanaka A
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 11 Pages: 1709-1712
  • DOI: 10.1292/jvms.16-0258
  • NAID: 130005170602
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Skin pH is the Master Switch of Kallikrein 5-Mediated Skin Barrier Destruction in a Murine Atopic Dermatitis Model. (2016)
  • Author(s): Jang H, Matsuda A, Jung K, Karasawa K, Matsuda K, Oida K, Ishizaka S, Ahn G, Amagai Y, Moon C, Kim SH, Arkwright PD, Takamori K, Matsuda H, Tanaka A.
  • Journal Title: J. Invest. Dermatol. Volume: 136 Issue: 1 Pages: 127-136
  • DOI: 10.1038/jid.2015.363
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dieckol, a phlorotannin of Ecklonia cava, suppresses IgE-mediated mast cell activation and passive cutaneous anaphylactic reaction (2015)
  • Author(s): Ahn G, Amagai Y, Matsuda A, Kang S-M, Lee W-W, Jung K, Oida K, Jang H, Ishizaka S, Matsuda K, Jeon Y-J, Jee Y, Matsuda H, Tanaka A
  • Journal Title: Experimental Dermatology Volume: 24 Issue: 12 Pages: 968-979
  • DOI: 10.1111/exd.12814
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Remarks] 獣医分子病態治療学研究室ホームページ
  • URL: http://web.tuat.ac.jp/~mol_path/
• [Remarks] 東京農工大学 獣医分子病態治療学研究室
  • URL: http://web.tuat.ac.jp/~mol_path/activity.html