| Project/Area Number |
15K14868
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Matsuda Hiroshi 東京農工大学, (連合)農学研究科(研究院), 教授 (80145820)
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| Research Collaborator |
ISHIZAKA Saori
SATO Hiroaki
TOISHI Yuko
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経成長因子 / ウマ / ストレス / 診断マーカー / サイトカイン / 輸送ストレス / クローニング / 相同性 / 測定系 |
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| Outline of Final Research Achievements |
In this study, I focused on nerve growth factor (NGF) to clarify the etiology and diagnostic significance in horse diseases secondary to the transportation stress. In addition, I attempted to establish an assay system for horse NGF as the biomarker. The results are as follows: 1) the sequence of horse NGF was identified and homology was more than 90% in comparison to the other animals. 2) an ELISA system for horse NGF was developed with a commercial antibody. 3) NGF levels were increased in peripheral blood of transportation-stressed horses using the established ELISA. Thus, I established a NGF assay system and usefulness of the NGF in the horse was confirmed.
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