Foot and mouth disease virus specific translation inhibition and application for development of disease resistant livestock

• Project/Area Number: 15K14871
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: Kohara Kyoko 鹿児島大学, 農水産獣医学域獣医学系, 教授 (20225478)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: FMDV / IRES / 宿主因子 / PTB / 4E-BP1 / ITAF45 / 細胞株 / CPK細胞 / MDBK細胞 / MDCK細胞

Outline of Final Research Achievements

FMD is a highly contagious infectious disease in cloven-hoofed animals. FMDV possess a positive sense, single stranded RNA genome. Internal ribosomal entry site (IRES) element exists within its 5′ UTR of the viral RNA. IRES mediated translation is unique translation mechanism, which are mostly observed in picornaviruses and hepacivirus. Our data indicated that IRES-mediated translational activity was not linked to FMDV host range. ITAF45 promoted IRES-mediated translation in all cell lines, and the effects of poly-pyrimidine tract binding protein (PTB) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) were observed only in FMDV-susceptible cells. Thus, PTB and 4E-BP1 may influence the host range of FMDV. Moreover, we have established the cell line expressing FMDV-IRES reporter gene, which can be applicable for the screening of FMDV-IRES inhibitors. Thus, these findings may contribute the development of disease resistant domestic animals and novel drugs.

Research Products

• [Journal Article] Susceptibility and initial immune response of Tupaia belangeri cells to dengue virus infection (2017)
  • Author(s): Kayesh MEH, Kitab B, Sanada T, Hayasaka D, Morita K, Kohara M, *Tsukiyama-Kohara K.
  • Journal Title: Infect Genet Evol. Volume: 51 Pages: 203-210
  • DOI: 10.1016/j.meegid.2017.04.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] IRES-mediated translation of foot-and-mouth disease virus (FMDV) in cultured cells derived from FMDV-susceptible and -insusceptible animals (2016)
  • Author(s): T. Kanda, M. Ozawa, K. Tsukiyama-Kohara
  • Journal Title: BMC Vet Res Volume: 12 Issue: 1 Pages: 66-66
  • DOI: 10.1186/s12917-016-0694-8
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of an intermittent cold stress model using <i>Tupaia belangeri</i> and evaluation of compound C737 targeting neuron-restrictive silencer factor (2016)
  • Author(s): Hai-Ying C, Nagano K, Ezzikouri S, Yamaguchi C, Kayesh ME, Rebbani K, Kitab B, Nakano H, Kouji H, Kohara M, Tsukiyama-Kohara K.
  • Journal Title: Experimental Animals Volume: 65 Issue: 3 Pages: 285-292
  • DOI: 10.1538/expanim.15-0123
  • NAID: 130006882368
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around Moukalaba-Doudou National Park (Gabon). (2016)
  • Author(s): Nze-Nkogue C, Horie M, Fujita S, Ogino M, Kobayashi Y, Mizukami K, Masatani T, Ezzikouri S, Matsuu A, Mizutani T, Ozawa M, Yamato O, Ngomanda A, Yamagiwa J, Tsukiyama-Kohara K
  • Journal Title: Virus Genes Volume: 52 Issue: 5 Pages: 671-678
  • DOI: 10.1007/s11262-016-1360-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] HCV-Induced Oxidative Stress: Battlefield-Winning Strategy. (2016)
  • Author(s): Rebbani K, Tsukiyama-Kohara K.
  • Journal Title: Oxid Med Cell Longev. Volume: 2016 Issue: 1 Pages: 7425628-7425628
  • DOI: 10.1155/2016/7425628
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Supplementing Conventional Treatment with Pycnogenol May Improve Hepatitis C Virus-Associated Type 2 Diabetes: A Mini Review. (2016)
  • Author(s): Ezzikouri S, Jadid FZ, Hamdi S, Wakrim L, Tsukiyama-Kohara K, Benjelloun S
  • Journal Title: J Clin Transl Hepatol. Volume: 4 Pages: 228-233
  • DOI: 10.14218/jcth.2016.00021
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Contribution of the interaction between the rabies virus P protein and I-kappa B kinase e; to the inhibition of type I IFN induction signalling. (2016)
  • Author(s): Masatani T, Ozawa M, Yamada K, Ito N, Horie M, Matsuu A, Okuya K, Tsukiyama-Kohara K, Sugiyama M, Nishizono A.
  • Journal Title: J Gen Virol. Volume: 97 Issue: 2 Pages: 316-326
  • DOI: 10.1099/jgv.0.000362
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Isolation and characterization of influenza A viruses from environmental water at an overwintering site of migratory birds in Japan. (2015)
  • Author(s): Okuya K, Kawabata T, Nagano K, Tsukiyama-Kohara K, Kusumoto I, Takase K, Ozawa M.
  • Journal Title: Arch Virol. Volume: 160 Issue: 12 Pages: 3037-3052
  • DOI: 10.1007/s00705-015-2610-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Full genome sequences of torque teno sus virus strains that coinfected a pig with postweaning multisystemic wasting syndrome in Japan: implications for genetic diversity. (2015)
  • Author(s): Ozawa M, Kawabata T, Okuya K, Nagano K, Kanda T, Kanazawa N, Tsukiyama-Kohara K, Taneno A, Deguchi E.
  • Journal Title: Arch Virol. Volume: 160 Issue: 12 Pages: 3067-3074
  • DOI: 10.1007/s00705-015-2593-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs. (2015)
  • Author(s): Miura R, Kooriyama T, Yoneda M, Takenaka A, Doki M, Goto Y, Sanjoba C, Endo Y, Fujiyuki T, Sugai A, Tsukiyama-Kohara K, Matsumoto Y, Sato H, Kai C.
  • Journal Title: PLoS Negl Trop Dis. Volume: 9 Issue: 7 Pages: e0003914-e0003914
  • DOI: 10.1371/journal.pntd.0003914
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic and serological surveillance for non-primate hepacivirus in horses in Japan (2015)
  • Author(s): Aya Matsuu, Seiji Hobo, Kunihide Ando, Takashi Sanekata, Fumio Sato, Yoshiro Endo, Tomohiko Amaya, Tomohiro Osaki, Masayuki Horie, Tatsunori Masatani, Makoto Ozawa, Kyoko Tsukiyama-Kohara
  • Journal Title: Veterinary Microbiology Volume: 179 Issue: 3-4 Pages: 219-227
  • DOI: 10.1016/j.vetmic.2015.05.028
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Efficient isolation of Swine influenza viruses by age-targeted specimen collection. (2015)
  • Author(s): Ozawa M, Matsuu A, Yonezawa K, Igarashi M, Okuya K, Kawabata T, Ito K, Tsukiyama-Kohara K, Taneno A, Deguchi E.
  • Journal Title: J Clin Microbiol. 2015 Apr;53(4):1331-8. Volume: 53 Issue: 4 Pages: 1331-1338
  • DOI: 10.1128/jcm.02941-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Remarks] TADセンター
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/TAD/index/php
• [Remarks] TADセンター
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/TAD/index.php
• [Patent(Industrial Property Rights)] 口蹄疫ウイルスIRES発現細胞 (2017)
  • Inventor(s): 小原 恭子
  • Industrial Property Rights Holder: 鹿児島大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017-05-31