Foot and mouth disease virus specific translation inhibition and application for development of disease resistant livestock
| Project/Area Number |
15K14871
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Kagoshima University |
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Principal Investigator |
Kohara Kyoko 鹿児島大学, 農水産獣医学域獣医学系, 教授 (20225478)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | FMDV / IRES / 宿主因子 / PTB / 4E-BP1 / ITAF45 / 細胞株 / CPK細胞 / MDBK細胞 / MDCK細胞 |
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| Outline of Final Research Achievements |
FMD is a highly contagious infectious disease in cloven-hoofed animals. FMDV possess a positive sense, single stranded RNA genome. Internal ribosomal entry site (IRES) element exists within its 5′ UTR of the viral RNA. IRES mediated translation is unique translation mechanism, which are mostly observed in picornaviruses and hepacivirus. Our data indicated that IRES-mediated translational activity was not linked to FMDV host range. ITAF45 promoted IRES-mediated translation in all cell lines, and the effects of poly-pyrimidine tract binding protein (PTB) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) were observed only in FMDV-susceptible cells. Thus, PTB and 4E-BP1 may influence the host range of FMDV. Moreover, we have established the cell line expressing FMDV-IRES reporter gene, which can be applicable for the screening of FMDV-IRES inhibitors. Thus, these findings may contribute the development of disease resistant domestic animals and novel drugs.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Establishment of an intermittent cold stress model using <i>Tupaia belangeri</i> and evaluation of compound C737 targeting neuron-restrictive silencer factor2016
Author(s)
Hai-Ying C, Nagano K, Ezzikouri S, Yamaguchi C, Kayesh ME, Rebbani K, Kitab B, Nakano H, Kouji H, Kohara M, Tsukiyama-Kohara K.
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Journal Title
Exp. Anim.
Volume: 65
Issue: 3
Pages: 285-292
DOI
NAID
ISSN
1341-1357, 1881-7122
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around Moukalaba-Doudou National Park (Gabon)2016
Author(s)
Nze-Nkogue C, Horie M, Fujita S, Ogino M, Kobayashi Y, Mizukami K, Masatani T, Ezzikouri S, Matsuu A, Mizutani T, Ozawa M, Yamato O, Ngomanda A, Yamagiwa J, Tsukiyama-Kohara K
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Journal Title
Virus Genes
Volume: 52
Pages: 671-678
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Efficacy of recombinant distemper virus expressing Leishmania Antigen against Leishmania challenge in dogs.2015
Author(s)
Miura R, Kooriyama T, Yoneda M, Takenaka A, Doki M, Goto Y, Sanjoba C, Endo Y, Fujiyuki T, Sugai A, Tsukiyama-Kohara K, Matsumoto Y, Sato H, Kai C.
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Journal Title
PLoS Negl Trop Dis
Volume: 9
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Genetic and serological surveillance for non-primate hepacivirus in horses in Japan.2015
Author(s)
Aya Matsuu, Seiji Hobo, Kunihide Ando, Takashi Sanekata, Fumio Sato, Yoshiro Endo, Tomohiko Amaya, Tomohiro Osaki, Masayuki Horie, Tatsunori Masatani, Makoto Ozawa, Kyoko Tsukiyama-Kohara
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Journal Title
Vet Microbiol
Volume: 179
Pages: 219-227
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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