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Foot and mouth disease virus specific translation inhibition and application for development of disease resistant livestock

Research Project

Project/Area Number 15K14871
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionKagoshima University

Principal Investigator

Kohara Kyoko  鹿児島大学, 農水産獣医学域獣医学系, 教授 (20225478)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsFMDV / IRES / 宿主因子 / PTB / 4E-BP1 / ITAF45 / 細胞株 / CPK細胞 / MDBK細胞 / MDCK細胞
Outline of Final Research Achievements

FMD is a highly contagious infectious disease in cloven-hoofed animals. FMDV possess a positive sense, single stranded RNA genome. Internal ribosomal entry site (IRES) element exists within its 5′ UTR of the viral RNA. IRES mediated translation is unique translation mechanism, which are mostly observed in picornaviruses and hepacivirus. Our data indicated that IRES-mediated translational activity was not linked to FMDV host range. ITAF45 promoted IRES-mediated translation in all cell lines, and the effects of poly-pyrimidine tract binding protein (PTB) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) were observed only in FMDV-susceptible cells. Thus, PTB and 4E-BP1 may influence the host range of FMDV. Moreover, we have established the cell line expressing FMDV-IRES reporter gene, which can be applicable for the screening of FMDV-IRES inhibitors. Thus, these findings may contribute the development of disease resistant domestic animals and novel drugs.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015 Other

All Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 12 results,  Open Access: 9 results,  Acknowledgement Compliant: 9 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Susceptibility and initial immune response of Tupaia belangeri cells to dengue virus infection2017

    • Author(s)
      Kayesh MEH, Kitab B, Sanada T, Hayasaka D, Morita K, Kohara M, *Tsukiyama-Kohara K.
    • Journal Title

      Infect Genet Evol.

      Volume: 51 Pages: 203-210

    • DOI

      10.1016/j.meegid.2017.04.003

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] IRES-mediated translation of foot-and-mouth disease virus (FMDV) in cultured cells derived from FMDV-susceptible and -insusceptible animals2016

    • Author(s)
      T. Kanda, M. Ozawa, K. Tsukiyama-Kohara
    • Journal Title

      BMC Vet Res

      Volume: 12 Issue: 1 Pages: 66-66

    • DOI

      10.1186/s12917-016-0694-8

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of an intermittent cold stress model using <i>Tupaia belangeri</i> and evaluation of compound C737 targeting neuron-restrictive silencer factor2016

    • Author(s)
      Hai-Ying C, Nagano K, Ezzikouri S, Yamaguchi C, Kayesh ME, Rebbani K, Kitab B, Nakano H, Kouji H, Kohara M, Tsukiyama-Kohara K.
    • Journal Title

      Experimental Animals

      Volume: 65 Issue: 3 Pages: 285-292

    • DOI

      10.1538/expanim.15-0123

    • NAID

      130006882368

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around Moukalaba-Doudou National Park (Gabon).2016

    • Author(s)
      Nze-Nkogue C, Horie M, Fujita S, Ogino M, Kobayashi Y, Mizukami K, Masatani T, Ezzikouri S, Matsuu A, Mizutani T, Ozawa M, Yamato O, Ngomanda A, Yamagiwa J, Tsukiyama-Kohara K
    • Journal Title

      Virus Genes

      Volume: 52 Issue: 5 Pages: 671-678

    • DOI

      10.1007/s11262-016-1360-8

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] HCV-Induced Oxidative Stress: Battlefield-Winning Strategy.2016

    • Author(s)
      Rebbani K, Tsukiyama-Kohara K.
    • Journal Title

      Oxid Med Cell Longev.

      Volume: 2016 Issue: 1 Pages: 7425628-7425628

    • DOI

      10.1155/2016/7425628

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Supplementing Conventional Treatment with Pycnogenol May Improve Hepatitis C Virus-Associated Type 2 Diabetes: A Mini Review.2016

    • Author(s)
      Ezzikouri S, Jadid FZ, Hamdi S, Wakrim L, Tsukiyama-Kohara K, Benjelloun S
    • Journal Title

      J Clin Transl Hepatol.

      Volume: 4 Pages: 228-233

    • DOI

      10.14218/jcth.2016.00021

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Contribution of the interaction between the rabies virus P protein and I-kappa B kinase e; to the inhibition of type I IFN induction signalling.2016

    • Author(s)
      Masatani T, Ozawa M, Yamada K, Ito N, Horie M, Matsuu A, Okuya K, Tsukiyama-Kohara K, Sugiyama M, Nishizono A.
    • Journal Title

      J Gen Virol.

      Volume: 97 Issue: 2 Pages: 316-326

    • DOI

      10.1099/jgv.0.000362

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Isolation and characterization of influenza A viruses from environmental water at an overwintering site of migratory birds in Japan.2015

    • Author(s)
      Okuya K, Kawabata T, Nagano K, Tsukiyama-Kohara K, Kusumoto I, Takase K, Ozawa M.
    • Journal Title

      Arch Virol.

      Volume: 160 Issue: 12 Pages: 3037-3052

    • DOI

      10.1007/s00705-015-2610-0

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Full genome sequences of torque teno sus virus strains that coinfected a pig with postweaning multisystemic wasting syndrome in Japan: implications for genetic diversity.2015

    • Author(s)
      Ozawa M, Kawabata T, Okuya K, Nagano K, Kanda T, Kanazawa N, Tsukiyama-Kohara K, Taneno A, Deguchi E.
    • Journal Title

      Arch Virol.

      Volume: 160 Issue: 12 Pages: 3067-3074

    • DOI

      10.1007/s00705-015-2593-x

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs.2015

    • Author(s)
      Miura R, Kooriyama T, Yoneda M, Takenaka A, Doki M, Goto Y, Sanjoba C, Endo Y, Fujiyuki T, Sugai A, Tsukiyama-Kohara K, Matsumoto Y, Sato H, Kai C.
    • Journal Title

      PLoS Negl Trop Dis.

      Volume: 9 Issue: 7 Pages: e0003914-e0003914

    • DOI

      10.1371/journal.pntd.0003914

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetic and serological surveillance for non-primate hepacivirus in horses in Japan2015

    • Author(s)
      Aya Matsuu, Seiji Hobo, Kunihide Ando, Takashi Sanekata, Fumio Sato, Yoshiro Endo, Tomohiko Amaya, Tomohiro Osaki, Masayuki Horie, Tatsunori Masatani, Makoto Ozawa, Kyoko Tsukiyama-Kohara
    • Journal Title

      Veterinary Microbiology

      Volume: 179 Issue: 3-4 Pages: 219-227

    • DOI

      10.1016/j.vetmic.2015.05.028

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Efficient isolation of Swine influenza viruses by age-targeted specimen collection.2015

    • Author(s)
      Ozawa M, Matsuu A, Yonezawa K, Igarashi M, Okuya K, Kawabata T, Ito K, Tsukiyama-Kohara K, Taneno A, Deguchi E.
    • Journal Title

      J Clin Microbiol. 2015 Apr;53(4):1331-8.

      Volume: 53 Issue: 4 Pages: 1331-1338

    • DOI

      10.1128/jcm.02941-14

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Remarks] TADセンター

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/TAD/index/php

    • Related Report
      2016 Annual Research Report
  • [Remarks] TADセンター

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/TAD/index.php

    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 口蹄疫ウイルスIRES発現細胞2017

    • Inventor(s)
      小原 恭子
    • Industrial Property Rights Holder
      鹿児島大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017-05-31
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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