Screening of candidate genes associating with the progression of chronic kidney disease in mice

• Project/Area Number: 15K14873
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Hokkaido University
• Principal Investigator: Ichii Osamu 北海道大学, 獣医学研究科, 准教授 (60547769)
• Co-Investigator(Renkei-kenkyūsha): HORINO TARO 高知大学, 医学部, 講師 (90448382)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 慢性腎臓病（CKD） / 嚢胞 / 原因遺伝子 / 遺伝子座 / ゲノム / 病理 / マウス / 遠位尿細管 / 獣医学

Outline of Final Research Achievements

Chronic kidney disease (CKD) is a serious problem from the points of increase of the patients with CKD as well as medical economy. In the present study, we discovered the candidate locus associating with the progression of CKD in DBA/2 mouse inbred strain. We named this locus as DBA/2 renal cyst“drecy”. From the pathological analysis, drecy locus related to the alternation of molecules associating with the transportations of ion or vesicle and cell-adhesion in the epithelial cells of distal tubules. We considered that these alternations would lead the abnormal phenotypes of distal tubules, including cyst formation. Furthermore, gene expression analysis suggested that the genes encoded on DBA/2-type chromosome 12 (D12Mit182-83), in particular Greb1, associate with the progression of these phenotypes.

Research Products

• [Journal Article] Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes (2016)
  • Author(s): Ichii O, Nakamura T, Irie T, Kouguchi H, Nakamura D, Nakamura S, Sato S, Yokoyama K, Horino T, Sunden Y, Elewa YH, Kon Y
  • Journal Title: Histochemistry and Cell Biology Volume: in press Issue: 3 Pages: 351-362
  • DOI: 10.1007/s00418-016-1438-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MRL/MpJ mice show unique pathological features after experimental kidney injury (2016)
  • Author(s): Shiozuru D, Ichii O, Kimura J, Nakamura T, Elewa YH, Otsuka-Kanazawa S, Kon Y
  • Journal Title: Histology and Histopathology Volume: 31(2) Pages: 189-204
  • Peer Reviewed
• [Presentation] マウス遠位尿細管の嚢胞形成に関与する新規原因遺伝子座の役割 (2016)
  • Author(s): 市居 修、中村 鉄平、矢吹 映、堀野 太郎、昆 泰寛
  • Organizer: 第159回 日本獣医学会学術集会
  • Place of Presentation: 神奈川県藤沢市日本大学
  • Year and Date: 2016-09-06
• [Presentation] 遠位尿細管上皮細胞の形態機能を制御するIL-1F6/IL-36α (2016)
  • Author(s): 市居 修、中村鉄平、岡村匡史、木村純平、堀野太郎、昆 泰寛
  • Organizer: 日本解剖学会第62回東北・北海道連合支部学術集会
  • Place of Presentation: 北海道帯広市帯広畜産大学
  • Year and Date: 2016-09-03
• [Presentation] 雌性コットンラット(Sigmodon hispidus)にみられる腎性貧血 (2016)
  • Author(s): 中村 鉄平, 市居 修, 入江 隆夫, 孝口 裕一, 寸田 祐嗣, 堀野 太郎, 昆 泰寛
  • Organizer: 第9回 日本獣医腎泌尿器学会学術集会
  • Place of Presentation: 東京都千代田区連合会館
• [Presentation] 遠位尿細管傷害を知らせるマーカー分子“IL-1F6/IL-36a”の有用性 (2015)
  • Author(s): 市居 修、中村 鉄平、堀野 太郎、木村 純平、岡村 匡史、昆 泰寛
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 青森県十和田市北里大学
  • Year and Date: 2015-09-09
• [Remarks] 北大・獣医・解剖学教室ホームページ
  • URL: https://www.vetmed.hokudai.ac.jp/organization/anat/
• [Remarks] 北海道大学研究者総覧
  • URL: https://researchers.general.hokudai.ac.jp/profile/ja.wkc9JlsishXmgpuI4vfbHA==.html
• [Remarks] 北大・獣医・解剖学教室ホームページ
  • URL: http://www.vetmed.hokudai.ac.jp/organization/anat/
• [Remarks] 北海道大学 研究者総覧
  • URL: http://researchers.general.hokudai.ac.jp/profile/ja.wkc9JlsishXmgpuI4vfbHA==.html