| Project/Area Number |
15K14874
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
LIU Qinghua 筑波大学, 国際統合睡眠医科学研究機構, 教授 (90723792)
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| Co-Investigator(Kenkyū-buntansha) |
曹 麗琴 筑波大学, 国際統合睡眠医科学研究機構, 助教 (60399475)
クレウエ・ネベニウス ダニエラ 筑波大学, 国際統合睡眠医科学研究機構, 研究員 (60737667)
佐藤 牧人 筑波大学, 国際統合睡眠医科学研究機構, 研究員 (70743699)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYAKAWA Reiko 公益財団法人大阪バイオサイエンス研究所, 神経機能学部門, 研究部長 (40372411)
YANAGISAWA Masashi 筑波大学, 国際統合睡眠医科学研究機構, 教授 (20202369)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | フォワード・ジェネティックス / 先天的恐怖 / 精神障害 / モデルマウス / バイオリソース / 恐怖情動 |
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| Outline of Final Research Achievements |
To understand the molecular mechanism of fear, we developed a robust predator odor-based innate fear assay, and conducted a forward genetic “FEAR” screen in N-ethyl-N-nitrosourea (ENU)-mutagenized mice. To date, we have successfully established two mutant pedigrees with abnormal fear behaviors, and identified the causative mutation in one mutant pedigree named Popcorn. We recreated Popcorn mutant mice by CRISPR/Cas9 system to confirm the causality of the Popcorn mutation. Moreover, we discovered that the Popcorn mice also exhibited socially submissive and anxiety phenotypes.
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