Development of generation method of knockout animal without using germ cells
| Project/Area Number |
15K14884
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Integrative animal science
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Fujii Wataru 東京大学, 大学院農学生命科学研究科(農学部), 助教 (40708161)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | ゲノム改変動物 / 発生工学 |
|---|
| Outline of Final Research Achievements |
In order to develop a method for preparing a transgenic animal without taking the germ cells out of the body, we studied the development of engineered endonucleases suitable for molecular delivery and the possibility of delivery via cell transplantation. First, we developed a system capable of expressing Cas9 and guide RNA with a single promoter. Besides, we developed a ZFN that functions efficiently, and also reported that S. thermophiles-derived orthologous CRISPR/Cas can be used for genome modification via zygotes. On the other hand, molecular delivery to recipient body and germ cell via transplanted cells was extremely limited, suggesting that it is hard to generate the genome-modified germ cells by delivery of engineered endonucleases.
|
Report
(3 results)
Research Products
(22 results)
