| Project/Area Number |
15K14886
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 魚類 / 卵子 / 凍結保存 / ゼブラフィッシュ |
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| Outline of Final Research Achievements |
Fish oocytes have not been cryopreserved successfully. We tried to vitrify immature zebrafish oocytes (Stage I and Stage III) expressed with an antifreeze protein and aquaporin 3, a water/cryoprotectant channel, and treated with ruthenium red. Ruthenium red is a nonspecific inhibitor of TRP channels and thus expected to inhibit hyperosmolarity-sensitive TRP channels. Without the expression and treatment, no oocytes survived just after warming, regardless of the growing stage. With the expression and treatment, no Stage I oocytes survived just after warming, but some Stage III oocytes survived. After 1 h of culture, however, the Stage III oocytes were not alive. Further studies are needed for successful cryopreservation of fish oocytes.
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