| Project/Area Number |
15K14887
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kyoto University (2017)
University of Miyazaki (2015-2016) |
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Principal Investigator |
Honda Arata 京都大学, 医学研究科, 特定准教授 (10373367)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | iPS細胞 / キメラ / 異種間キメラ / ES細胞 / ナイーブ型 / 多能性幹細胞 / ナイーブ / プライム |
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| Outline of Final Research Achievements |
This research project had been aimed for the derivation of cynomolgus monkey/rabbit interspecific chimera using blastocyst complementation methods. We achieved naive-like conversion of cynomolgus monkey ES/iPS cells, which can enhances their in vitro differentiation potential. According to the research project, it revealed that rabbit embryos were not suitable for the host of interspecific chimera production. On the other hand, following mice and rats, the 3rd true naive-iPS cells were successfully established form an endangered species, Tokudaia osimensis. We produced interspecific chimera and elucidated the sexual plasticity of the germ cells of the endangered species.
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