Rabbit/Cynomolgus monkey interspecific chimera produced by blastocyst complementation.

• Project/Area Number: 15K14887
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Kyoto University (2017); University of Miyazaki (2015-2016)
• Principal Investigator: Honda Arata 京都大学, 医学研究科, 特定准教授 (10373367)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: iPS細胞 / キメラ / 異種間キメラ / ES細胞 / ナイーブ型 / 多能性幹細胞 / ナイーブ / プライム

Outline of Final Research Achievements

This research project had been aimed for the derivation of cynomolgus monkey/rabbit interspecific chimera using blastocyst complementation methods. We achieved naive-like conversion of cynomolgus monkey ES/iPS cells, which can enhances their in vitro differentiation potential. According to the research project, it revealed that rabbit embryos were not suitable for the host of interspecific chimera production. On the other hand, following mice and rats, the 3rd true naive-iPS cells were successfully established form an endangered species, Tokudaia osimensis. We produced interspecific chimera and elucidated the sexual plasticity of the germ cells of the endangered species.

Research Products

• [Journal Article] Applying iPSCs for preserving endangered species and elucidating the evolution of mammalian sex determination (2018)
  • Author(s): Arata Honda
  • Journal Title: BioEssays Volume: 印刷中 Issue: 6 Pages: 1700152-1700152
  • DOI: 10.1002/bies.201700152
  • Peer Reviewed / Open Access
• [Journal Article] Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis (2017)
  • Author(s): Arata Honda, Narantsog Choijookhuu, Haruna Izu, Yoshihiro Kawano, Mizuho Inokuchi, Kimiko Honsho, Ah-Reum Lee, Hiroki Nabekura, Hiroshi Ohta, Tomoyuki Tsukiyama, Yasuhide Ohinata, Asato Kuroiwa, Yoshitaka Hishikawa, Mitinori Saitou, Takamichi Jogahara, Chihiro Koshimoto
  • Journal Title: Science Advances Volume: - Issue: 5
  • DOI: 10.1126/sciadv.1602179
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Discrimination of stem cell status after subjecting cynomolgus monkey pluripotent stem cells to naive conversion. (2017)
  • Author(s): Honda A, Kawano Y, Izu H, Choijookhuu N, Honsho K, Nakamura T, Yabuta Y, Yamamoto T, Takashima Y, Hirose M, Sankai T, Hishikawa Y, Ogura A, Saitou M
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 45285-45285
  • DOI: 10.1038/srep45285
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Flexible adaptation of male germ cells from female iPS cells of endangered Tokudaia osimensis (2017)
  • Author(s): Arata Honda, Narantsog Choijookhuu, Haruna Izu, Yoshihiro Kawano, Mizuho Inokuchi, Kimiko Honsho, Ah-Reum Lee, Hiroki Nabekura, Hiroshi Ohta, Tomoyuki Tsukiyama, Yasuhide Ohinata, Asato Kuroiwa, Yoshitaka Hishikawa, Mitinori Saitou, Takamichi Jogahara, Chihiro Koshimoto.
  • Journal Title: Science Advances Volume: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 絶滅危惧種の特徴を iPS 細胞と発生工学で解き明かす (2018)
  • Author(s): 本多 新
  • Organizer: 関西実験動物研究会
  • Invited
• [Presentation] Germ Cells from Induced Pluripotent Stem Cells of an Endangered Species, Tokudaia Osimensis (2017)
  • Author(s): Arata Honda, Narantsog Choijookhuu, Haruna Izu, Yoshihiro Kawano, Yoshitaka Hishikawa, Takamichi Jogahara, Chihiro Koshimoto.
  • Organizer: Fourth World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] メスの細胞から精子｜性染色体XO型の絶滅危惧種アマミトゲネズミ細胞の性的柔軟性 (2017)
  • Author(s): 本多 新
  • Organizer: ConBio 2017
  • Invited
• [Presentation] iPS細胞と医療を繋ぐ (2017)
  • Author(s): 本多 新
  • Organizer: 日本看護遺伝学会第16回学術大会
  • Invited
• [Remarks] 宮崎大学テニュアトラック推進機構 本多研究室
  • URL: http://www.cc.miyazaki-u.ac.jp/maruhon/index.html