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Identification of hormone-dependent microRNA that regulates sexual development of insects

Research Project

Project/Area Number 15K14892
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Insect science
Research InstitutionThe University of Tokyo

Principal Investigator

Suzuki Masataka  東京大学, 大学院新領域創成科学研究科, 准教授 (30360572)

Research Collaborator AOKI Fugaku  
KATAOKA Hiroshi  
MATSUNO Kumiko  
MATSUSHIMA Daijiro  
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords性決定 / 性分化 / 性ホルモン / エクダイソン / microRNA / カイコ / エクダイソン受容体EcR / ポナステロン / doublesex / 胚発生 / Bmdsx / エクダイソン受容体 / EPPase
Outline of Final Research Achievements

To investigate whether ecdysone is involved in sexual development of the silkworm in the sex-determining embryonic stage, we compared the amount of 20-hydroxyecdysone and expression levels of genes involved in ecdysone synthesis and ecdysone signaling between male and female embryos. Although these levels showed no sexual difference, knockdown of EPP-ase, which is required for ecdysone synthesis during embryonic stages, reduced expression level of Bmdsx in male and female embryos. In addition, knockdown of the ecdysone receptor gene EcR also caused decreased expression of Bmdsx in embryos. These results demonstrate that ecdysone signaling stimulates Bmdsx expression in the embryonic stages.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) Remarks (3 results)

  • [Journal Article] Identification of the Doublesex protein binding sites that activate expression of lozenge in the female genital disc in Drosophila melanogaster2017

    • Author(s)
      Wagamitsu S, Takase D, Aoki F, and Suzuki MG.
    • Journal Title

      Mechanism of Development

      Volume: 143 Pages: 26-31

    • DOI

      10.1016/j.mod.2017.01.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] カイコの<i>doublesex</i>の機能とその性特異的スプライシング制御機構2015

    • Author(s)
      鈴木雅京
    • Journal Title

      Sanshi-Konchu Biotec

      Volume: 84 Issue: 1 Pages: 1_25-1_35

    • DOI

      10.11416/konchubiotec.84.1_25

    • NAID

      130005087596

    • ISSN
      1881-0551, 1884-7943
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] カブラハバチのdoublesexオルソログの同定とその機能解析2017

    • Author(s)
      峰 翔太郎, 畠山正統, 炭谷めぐみ, 青木不学, 鈴木雅京
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都小金井市)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] ショウジョウバエの雄生殖原基におけるbranchlessの発現に必要なDSX結合シスエレメントの探索2017

    • Author(s)
      澤本東海, 青木不学, 鈴木雅京
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都小金井市)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] カイコのDMRT遺伝子の発現および機能解析2017

    • Author(s)
      笠原良太, 青木不学, 鈴木雅京
    • Organizer
      日本蚕糸学会第87回大会
    • Place of Presentation
      農林水産技術会議事務局筑波産学連携支援センター(茨城県つくば市)
    • Year and Date
      2017-03-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] イエバエの雌決定遺伝子transformerは自己の雌型スプライシングを制御する2017

    • Author(s)
      田中有沙, 青木不学, 鈴木雅京
    • Organizer
      日本蚕糸学会第87回大会
    • Place of Presentation
      日本蚕糸学会第87回大会
    • Year and Date
      2017-03-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] カイコ胚子期におけるecdysone量及びecdysone関連遺伝子発現量の性差解析2016

    • Author(s)
      松島 大二朗、青木 不学、鈴木 雅京
    • Organizer
      平成28年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      京都工芸繊維大学
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report
  • [Presentation] E75 regulates the steroid-hormone induced expression of let-7 microRNA in the silkworm, Bombyx mori2015

    • Author(s)
      Saowaros Chorach, Masataka G. Suzuki, Kumiko Matsuno
    • Organizer
      第38回日本分子生物学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Remarks] 資源生物制御学分野ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/seigyo/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東京大学大学院新領域創成科学研究科 先端生命科学専攻ホームページ

    • URL

      http://www.ib.k.u-tokyo.ac.jp/

    • Related Report
      2015 Research-status Report
  • [Remarks] 東京大学大学院新領域創成科学研究科 先端生命科学専攻 資源生物制御学分野ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/seigyo/

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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