| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Wolbachia is an endosymbiotic bacterium that infects a wide range of insect species. Infection of Ostrinia furnacalis by Wolbachia causes the production of all-female progeny. In the present study, we performed RNA-seq analysis of Wolbachia-infected and Wolbachia-uninfected Ostrinia embryos and revealed that Wolbachia infection markedly reduces the mRNA level of Masc, a gene that encodes a protein required for both masculinization and dosage compensation. Further bioinformatic analysis elucidated that dosage compensation of Z-linked genes fails in Wolbachia-infected Ostrinia embryos. In addition, injection of Masc cRNA into Wolbachia-infected embryos rescued male progeny. These results show that Wolbachia-induced male-killing is caused by a failure of dosage compensation via repression of the mRNA level of the host masculinizing gene Masc.
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