Protein production using Daphnia magna
| Project/Area Number |
15K14897
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ミジンコ / タンパク質産生 / ゲノム編集 / ビテロジェニン / 細胞間輸送 / シグナルペプチド |
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| Outline of Final Research Achievements |
We aimed to produce artificial protein in Daphnia magna using genome editing technique. Firstly we made a synthetic gene that has a signal peptide necessary for secretion with green fluorescent protein (GFP) and confirmed that the product of the synthetic gene was secreted into hemolymph. Secondly we fused a gene fragment responsible for incorporation into eggs with the synthetic gene. This gene product was successfully synthesized in somatic cells ant transported to eggs.
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Report
(3 results)
Research Products
(35 results)
