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Assignment of the nitrogen fixed ability to a plant by use of the endophyte- bacteria symbiosis system.

Research Project

Project/Area Number 15K14902
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental agriculture(including landscape science)
Research InstitutionIbaraki University

Principal Investigator

Narisawa Kazuhiko  茨城大学, 農学部, 教授 (90431650)

Co-Investigator(Kenkyū-buntansha) 西澤 智康  茨城大学, 農学部, 准教授 (40722111)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsエンドファイト / 内生バクテリア / 共生 / 窒素固定 / 植物生育促進
Outline of Final Research Achievements

The objective of the study is to clarify the interaction of Veronaeopsis simplexY34 and Rhizobium sp. Y9. Therefore, we did the bacterial comparative genome analysis and the inoculation experiment to tomato plant. The gene cluster in regard of a nitrogen fixation or a root nodule formation did not exist in the circle plasmid of Rhizobium sp. Y9. Rhizobium sp. Y9 did not show the growth promoting effect to the plant by a nitrogen fixation. However, Rhizobium sp. Y9 improved the infection rate to the plant with V. simplexY34.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Mycoavidus cysteinexigens gen. nov., sp. nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongata.2016

    • Author(s)
      Ohshima S, Sato Y, Fujimura R, Takashima Y, Hamada M, Nishizawa T, Narisawa K, Ohta H.
    • Journal Title

      International journal of systematic and evolutionary microbiology

      Volume: 66 Issue: 5 Pages: 2052-2057

    • DOI

      10.1099/ijsem.0.000990

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] <i>Cladophialophora inabaensis</i> sp. nov., a New Species among the Dark Septate Endophytes from a Secondary Forest in Tottori, Japan2016

    • Author(s)
      Usui E, Takashima Y, Narisawa K
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 3 Pages: 357-360

    • DOI

      10.1264/jsme2.ME16016

    • NAID

      130005265059

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 土壌微生物が創る共生の世界-その先端的研究事例と農業への応用的研究展開,4.糸状菌に共生する細菌その機能と生態-2016

    • Author(s)
      太田寛行・西澤智康・松岡勇人・高島勇介・成澤才彦
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 87 Pages: 254-259

    • NAID

      130005966784

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 糸状菌,特にエンドファイトの諸形質を内生細菌がコントロールするのか?2015

    • Author(s)
      高島勇介,太田寛之,成澤才彦
    • Journal Title

      土と微生物

      Volume: 69 Pages: 16-24

    • NAID

      110009986655

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Presentation] A dark septate endophyte Veronaeopsis simplex Y34 alters the root-endospheric community and suppresses Fusarium crown and root rot disease of tomato.2016

    • Author(s)
      Guo Y., Nishizawa T., Ohta H. and Narisawa K.
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀文化会館
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 真菌類菌糸圏から分離したBurkholderia属細菌のキチン分解活性について2016

    • Author(s)
      中西 布実子、高島 勇介、菊池 義智、出川 洋介、成澤 才彦
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀文化会館
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genome analysis of a DSE-commensal bacterium Rhizobium sp. Y9 associated with Veronaeopsis simplex Y34.2016

    • Author(s)
      Guo Y., Matsuoka Y., Nishizawa T., Ohta H. and Narisawa K.
    • Organizer
      日本土壌微生物学会2016年度大会
    • Place of Presentation
      岐阜大学
    • Year and Date
      2016-06-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラン菌根菌は内生バクテリアを保持するのか2015

    • Author(s)
      中西布実子、成澤 才彦
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      茨城 土浦市
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Isolation and selection of cellulolytic dark septate endophytic fungi that able to promote asparagus plant growth.2015

    • Author(s)
      Surono and Kazuhiko Narisawa
    • Organizer
      The 7th Japan-Taiwan-Korea International Symposium on Microbial Ecology
    • Place of Presentation
      Tsuchiura, Japan
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] グロムス門およびケカビ亜門菌類に内生するバクテリアの多様性2015

    • Author(s)
      高島 勇介、成澤 才彦
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      茨城 土浦市
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Detection of Mollicutes- related endobacteria from putative saprotrophic Endogone spp. and Sphaerocreas pubescens2015

    • Author(s)
      TAKASHIMA, Y., K. Yamamoto, K. Seto, Y. Degawa and K. Narisawa
    • Organizer
      The 8th International Conference on Mycorrhiza
    • Place of Presentation
      Flagstaff, Arizona, USA
    • Year and Date
      2015-08-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Endogone属菌およびSphaerocreas pubescensにおけるMollicutes-related endobacteriaの検出2015

    • Author(s)
      高島勇介、山本航平、瀬戸健介、出川洋介、成澤才彦
    • Organizer
      日本菌学会第59回大会
    • Place of Presentation
      沖縄 那覇市
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report
  • [Presentation] 塩害土壌におけるトマト栽培へのPhialocephala helveticaの利用2015

    • Author(s)
      臼井絵里香、菊地聖永、成澤才彦
    • Organizer
      日本菌学会第59回大会
    • Place of Presentation
      沖縄 那覇市
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report
  • [Presentation] 部エンドファイトVeronaeopsis simplexに内生するバクテリアについて2015

    • Author(s)
      松岡 勇人、Rida Khastini、成澤 才彦
    • Organizer
      日本菌学会第59回大会
    • Place of Presentation
      沖縄 那覇市
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report
  • [Presentation] 菌類子実体内部から分離されたBurkholderia属菌について2015

    • Author(s)
      中西布実子、成澤 才彦
    • Organizer
      日本菌学会第59回大会
    • Place of Presentation
      沖縄 那覇市
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report
  • [Presentation] 部エンドファイトPhialocephala fortinii BPC3によるトマトへのCs-137移行の抑制2015

    • Author(s)
      丸山剛、坂上伸生、小松崎将一、成澤才彦
    • Organizer
      日本菌学会第59回大会
    • Place of Presentation
      沖縄 那覇市
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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