| Project/Area Number |
15K14911
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
ABE Keietsu 東北大学, 農学研究科, 教授 (50312624)
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| Co-Investigator(Renkei-kenkyūsha) |
NANATANI Kei 東北大学, 大学院工学研究科, 助教 (00547333)
OGASAWARA Satoshi 東北大学, 医学系研究科, 助教 (30546685)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 輸送体 / 物質生産 / 構造解析 |
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| Outline of Final Research Achievements |
Aspartate:alanine antiporter AspT and its orthologues are industrially important solute exporters in bacteria. We established expression and purification systems for AspT with high yield and purity. We screened thermo-stable conditions for AspT in the presence of several ligands including substrates and their analogs. We performed crystallization of AspT and obtained some crystals at several optimized conditions. We collected X-ray diffraction data of AspT with the resolution of 4-6 Å, and are trying to improve molecular packing of crystals of AspT for better resolution.
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