| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Towards comprehensive design of chloroplast genomes in higher plants, in this work, we attempted to develop the method for allowing complete replacement of the wild-type plastid genomes with artificially constructed ones. Firstly, we established an engineered riboswitch-based inducible gene expression system for strict regulation of plastid genes. Then, we examined whether a riboswitch-controlled artificial endonuclease, exclusively recognizing specific DNA sequences derived from the wild-type plastid genomes, could help to achieve homoplasmy of transplastomic lines.
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