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Development of the method for generation of transplastomic plants harboring artificially designed plastid genomes

Research Project

Project/Area Number 15K14912
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionIbaraki University

Principal Investigator

NAKAHIRA Yoichi  茨城大学, 農学部, 准教授 (40423868)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords葉緑体 / 合成生物学 / 人工ゲノム / 遺伝子発現制御 / 人工リボスイッチ / 人工ヌクレアーゼ / 葉緑体ゲノム / 葉緑体形質転換 / 遺伝子発現誘導系 / 人工制限酵素 / 形質転換技術 / 形質転換
Outline of Final Research Achievements

Towards comprehensive design of chloroplast genomes in higher plants, in this work, we attempted to develop the method for allowing complete replacement of the wild-type plastid genomes with artificially constructed ones. Firstly, we established an engineered riboswitch-based inducible gene expression system for strict regulation of plastid genes. Then, we examined whether a riboswitch-controlled artificial endonuclease, exclusively recognizing specific DNA sequences derived from the wild-type plastid genomes, could help to achieve homoplasmy of transplastomic lines.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (10 results)

  • [Journal Article] Comparative analysis of chloroplast psbD promoters in terrestrial plants2017

    • Author(s)
      Shimmura S., Nozoe, M., Kitora, S., Kin S., Matsutani, S., Ishizaki, Y., Nakahira, Y. and *Shiina, T
    • Journal Title

      Front. in Plant Sci

      Volume: 8 Pages: 1186-1186

    • DOI

      10.3389/fpls.2017.01186

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria2015

    • Author(s)
      Sayaka Hondo, Masatoshi Takahashi, Takashi Osanai, Mami Matsuda, Tomohisa Hasunuma, Akio Tazuke, Yoichi Nakahira, Shigeru Chonan, Morifumi Hasegawa, Munehiko Asayama
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 120 Issue: 5 Pages: 510-517

    • DOI

      10.1016/j.jbiosc.2015.03.004

    • NAID

      110010017115

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Metabolic engineering for improved production of drop-in fuels in the cyanobacterium Synechococcus elongatus PCC 79422017

    • Author(s)
      Hiroki Kaneko, Hirofumi Fukuda, Munehiko Asayama, and Yoichi Nakahira
    • Organizer
      第58回 日本植物生理学会年会
    • Place of Presentation
      鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Utilization of a theophylline-dependent engineered riboswitch for enhanced alkane production in the cyanobacterium Synechocystis sp. PCC 68032017

    • Author(s)
      Hirofumi Fukuda, Hiroki Kaneko, Munehiko Asayama, and Yoichi Nakahira
    • Organizer
      第58回 日本植物生理学会年会
    • Place of Presentation
      鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 人工リボスイッチを活用した葉緑体遺伝子発現誘導系の評価2017

    • Author(s)
      山根 里佳、小川 敦司、戸澤 譲、椎名 隆、中平 洋一
    • Organizer
      2017年度 生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] テオフィリン依存型人工リボスイッチを用いたシアノバクテリアにおけるアルカン増産の試み2017

    • Author(s)
      金子 太樹、福田 寛史、武波 洋志、朝山 宗彦、中平 洋一
    • Organizer
      2017年度 生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 人工リボスイッチを活用した葉緑体遺伝子発現制御系の評価2017

    • Author(s)
      山根 里佳、小川 敦司、戸澤 譲、椎名 隆、中平 洋一
    • Organizer
      第35回 日本植物細胞分子生物学会(さいたま)大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シアノバクテリアにおけるドロップイン燃料生産性強化に向けた遺伝子改変技術の開発2016

    • Author(s)
      金子太樹、山根里佳、櫻井大貴、福田寛史、朝山宗彦、中平洋一
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] シアノバクテリアのアルカンと脂肪生産に与える栄養源欠乏培地の影響2016

    • Author(s)
      福田寛史、中平洋一、長谷川守文、宮口右二、朝山宗彦
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] 人工リボスイッチを基盤とした葉緑体遺伝子発現誘導系2016

    • Author(s)
      中平洋一、山根里佳、小川敦司、戸澤譲、椎名隆
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 人工リボスイッチを用いたタバコ葉緑体遺伝子発現誘導系2016

    • Author(s)
      中平洋一、小川敦司、戸澤譲、椎名隆
    • Organizer
      第34回 日本植物細胞分子生物学会大会
    • Place of Presentation
      上田
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] Utilization of the theophylline-dependent engineered riboswitches for strict control of plastid gene expression in tobacco.2016

    • Author(s)
      中平 洋一、小川敦司、戸澤譲、椎名隆
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学 上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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