Development of the method for generation of transplastomic plants harboring artificially designed plastid genomes
| Project/Area Number |
15K14912
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 葉緑体 / 合成生物学 / 人工ゲノム / 遺伝子発現制御 / 人工リボスイッチ / 人工ヌクレアーゼ / 葉緑体ゲノム / 葉緑体形質転換 / 遺伝子発現誘導系 / 人工制限酵素 / 形質転換技術 / 形質転換 |
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| Outline of Final Research Achievements |
Towards comprehensive design of chloroplast genomes in higher plants, in this work, we attempted to develop the method for allowing complete replacement of the wild-type plastid genomes with artificially constructed ones. Firstly, we established an engineered riboswitch-based inducible gene expression system for strict regulation of plastid genes. Then, we examined whether a riboswitch-controlled artificial endonuclease, exclusively recognizing specific DNA sequences derived from the wild-type plastid genomes, could help to achieve homoplasmy of transplastomic lines.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Comparative analysis of chloroplast psbD promoters in terrestrial plants2017
Author(s)
Shimmura S., Nozoe, M., Kitora, S., Kin S., Matsutani, S., Ishizaki, Y., Nakahira, Y. and *Shiina, T
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Journal Title
Front. in Plant Sci
Volume: 8
Pages: 1186-1186
DOI
Related Report
Peer Reviewed / Open Access
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