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Edible vaccine production using plastid transformation in plants.

Research Project

Project/Area Number 15K14913
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

ONO Michiyuki  筑波大学, 生命環境系, 准教授 (50201405)

Co-Investigator(Renkei-kenkyūsha) TAKEUCHI Kaoru  筑波大学, 医学医療系, 准教授 (00192162)
MORIKAWA Kazuya  筑波大学, 医学医療系, 教授 (90361328)
YASUTOMI Yasuhiro  国立研究開発法人医薬基盤・健康・栄養研究所, 霊長類医科学研究センター, センター長 (90281724)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords食べるワクチン / 新型インフルエンザ / E型肝炎ウイルス / ウイルス様粒子 / 色素体形質転換 / ニンジン / 不定胚 / タバコ / 遺伝子組換え作物 / 葉緑体形質転換 / 新型インフレンザ / 人工種子
Outline of Final Research Achievements

Virus-like particle (VLP) is a self-assembled capsid protein of virus and the best candidate of edible vaccine, because some VLP is resistant to digestion and can be targeted to the immune system of intestine. We tried to make transplastomic plants of carrot (Daucus carota L. cv. Kurodagosun) and tobacco (Nicotiana tabacum cv. SR-1) expressing capsid protein of Human Hepatitis E Virus (HEV) fused with the M2 universal epitope of Infuenza type A virus that will be an ideal edible vaccine. We could obtain candidate of transplastomic carrot embryogenic callus and homoplasmic tobacco plants. Some tobacco plant contains the vaccine protein that is enough concentration for immunogenicity feeding test of mice.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2016 Other

All Presentation (1 results) Remarks (2 results)

  • [Presentation] 葉緑体による新型インフルエンザウイルスに対する食べるワクチンの生産2016

    • Author(s)
      岡田 拓也,髙橋享介,小野公代,森川一也,竹内薫,保富康宏,小野道之
    • Organizer
      第34回日本植物細胞分子生物学会(上田)大会
    • Place of Presentation
      信州大学繊維学部(長野県上田市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Remarks] 植物発生生理学研究室ホームページ

    • URL

      http://gm-edu.sakura.ne.jp/labo/vaccin

    • Related Report
      2016 Annual Research Report
  • [Remarks] GM 作物を用いた食べるワクチンの開発に関する研究

    • URL

      http://gradtex.biol.tsukuba.ac.jp/2015/tjb201601/201413051.pdf

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-12-27  

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