Construction of a novel fluorescent measurement system to evaluate pH values in fluorescent imaging analysis

Research Project

Project/Area Number	15K14914
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Applied molecular and cellular biology
Research Institution	The University of Tokyo
Principal Investigator	MISAKA Takumi 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (40373196)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	培養細胞 / pH / 蛍光イメージング / ｐH
Outline of Final Research Achievements	We planned to construct a novel evaluation system to detect a decrease in pH value of extracellular solution by fluorescent imaging analysis. Extracellular pH decease could be clearly detected by the change of fluorescent intensities of EGFP by using cultured cells that express both a novel proton channel and EGFP. It was also shown that sensitivities of sensor cell lines against pH decrease depend on the expression level of a novel proton channel.