| Project/Area Number |
15K14917
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
SUGITA Mamoru 名古屋大学, 遺伝子実験施設, 教授 (70154474)
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| Co-Investigator(Renkei-kenkyūsha) |
ICHINOSE Mizuho 名古屋大学, トランスフォーマテイブ生命分子研究所, 特任助教 (60755718)
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| Research Collaborator |
SUGITA Chieko 名古屋大学, 遺伝子実験施設, 研究員 (30402457)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | RNA結合タンパク質 / PPRタンパク質 / RNAスプライシング / RNA編集 / RNA分解 / ヒメツリガネゴケ / 葉緑体 / ミトコンドリア / RNA結合モチーフ / NYNドメイン / 植物オルガネラ / RNA調節酵素 |
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| Outline of Final Research Achievements |
A new tool for manipulating organelle RNA functions urgently needs to be developed. Pentatricopeptide repeat (PPR) proteins are sequence-specific RNA-binding proteins and consist of a tandem array of multiple PPR motifs. Each of PPR motifs aligns to one nucleotide in the RNA target. The amino acid side chains at two specific positions in each motif confer nucleotide specificity in a predictable and programmable manner. Thus, PPR proteins provide an extremely promising opportunity to create custom RNA-binding PPR proteins with tailored specificity. In this study, we created custom PPR proteins to understand PPR-RNA recognition modes and to manipulate target RNA molecules.
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