| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Alternative mRNA splicing is a fundamental mechanism to produce plenty of protein more than those encoded in its genome. The understanding of alternative splicing will make us to develop the therapy strategy for diseases based on the inadequate miss-splicing.
CHERP is first identified as Ca2+ signaling related protein in the endoplasmic reticulum. Then, CHERP is also localized in the nucleus, suggesting CHERP has another function. Here, I examined the CHERP regulating genes and alternative splicing. To analyze the genome wide expression change of each mRNA and exon, the exon array analysis was performed. To know the function of CHERP, then, GO term analysis was also carried out. The depletion of CHERP caused the decreased expression of cell cycle, mitosis, cytokinesis, meiosis, DNA repair and DNA replication GO term and increased the induction of apoptosis GO term, suggesting that CHERP has a role for cell proliferation and survival by regulating mRNA splicing.
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