• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction of novel membrane-bound molecular probes for lytic granulces and their functional analysis

Research Project

Project/Area Number 15K14920
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionKyoto Institute of Technology

Principal Investigator

Kataoka Takao  京都工芸繊維大学, 応用生物学系, 教授 (20242307)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsperforin / granzyme B / LAMP1 / 細胞傷害顆粒 / eomesodermin / IFN-γ / SLC3A2 / interferon-γ / LAMP-1 / パーフォリン / グランザイムB
Outline of Final Research Achievements

HM13, TMED10, and SLC3A2 co-localized partly to LysoTracker Red DND-99, and perforin and granzyme B, which were fused to the C-terminal region of LAMP1, co-localized to LysoTracker Red DND-99 in human lung adenocarcinoma A549 cells. LAMP1 has been shown to localize mainly to the trans-Golgi network in Syrian hamster kidney BHK-21 cells. The T-box transcription factor eomesodermin has been shown to promote the binding of RelA and NFATc2 to the promoter region and multiple conserved noncoding sequences across the Ifng locus in mouse thymoma BW5147 cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2017 2015

All Presentation (3 results)

  • [Presentation] T-boxファミリー転写因子eomesoderminはマウス胸腺腫BW5147細胞においてT細胞の活性化に応答しIFN-γの発現を制御する2017

    • Author(s)
      原田 美鈴、福岡 菜月、西田 愛、片岡 孝夫
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] リソソーム関連タンパク質の細胞内局在性の解析2017

    • Author(s)
      家治 葉子、桑田 沙羅、片岡 孝夫
    • Organizer
      日本農芸化学会2017年度(平成29年度)大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Related Report
      2016 Research-status Report
  • [Presentation] The lysosomal membrane protein LAMP1 localizes mainly to trans-Golgi network in baby hamster kidney (BHK-21) cells2015

    • Author(s)
      桑田 沙羅、奥田 直明、西田 愛、福岡 菜月、片岡 孝夫
    • Organizer
      第38回 日本分子生物学会年会、第88回 日本生化学会 合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi