Construction of novel membrane-bound molecular probes for lytic granulces and their functional analysis
| Project/Area Number |
15K14920
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
Kataoka Takao 京都工芸繊維大学, 応用生物学系, 教授 (20242307)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | perforin / granzyme B / LAMP1 / 細胞傷害顆粒 / eomesodermin / IFN-γ / SLC3A2 / interferon-γ / LAMP-1 / パーフォリン / グランザイムB |
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| Outline of Final Research Achievements |
HM13, TMED10, and SLC3A2 co-localized partly to LysoTracker Red DND-99, and perforin and granzyme B, which were fused to the C-terminal region of LAMP1, co-localized to LysoTracker Red DND-99 in human lung adenocarcinoma A549 cells. LAMP1 has been shown to localize mainly to the trans-Golgi network in Syrian hamster kidney BHK-21 cells. The T-box transcription factor eomesodermin has been shown to promote the binding of RelA and NFATc2 to the promoter region and multiple conserved noncoding sequences across the Ifng locus in mouse thymoma BW5147 cells.
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Report
(4 results)
Research Products
(3 results)
