| Project/Area Number |
15K14921
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
Miura Daisuke 九州大学, 農学研究院, 特任准教授 (40532627)
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| Co-Investigator(Kenkyū-buntansha) |
藤村 由紀 九州大学, 農学研究院, 特任准教授 (20390304)
早川 英介 沖縄科学技術大学院大学, 進化神経生物学ユニット, 研究員 (20739809)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 質量分析 / イメージング / メタボロミクス / 質量分析イメージング / 代謝動態 / メタボローム / 植物代謝 |
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| Outline of Final Research Achievements |
We visualized the spatial distribution of metabolites within tissue sections of tomato fruit using a matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) technique. This technique elucidated the unique distribution patterns of more than 30 metabolite-derived ions, including primary and secondary metabolites, simultaneously. To investigate spatiotemporal metabolic alterations during physiological changes, MALDI-MSI was performed using the different ripening phenotypes. The amounts of glutamate and adenosine monophosphate, umami compounds, increased in both mesocarp and locule regions during the ripening process. MALDI-MSI was also applied to evaluate more local metabolic responses to wounding stress. Accumulations of a tomatine and a low level of its glycosylated metabolite, esculeoside A, were found in the wound region. Their inverse levels were observed in non-wounded regions. Furthermore, the amounts of both compounds differed in the developmental stages.
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