Building up the bioluminescence molecule on the nucleic acid sequence and its application to gene detection technology
| Project/Area Number |
15K14933
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical pharmacy
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KOJIMA Naoshi 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30356985)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 遺伝子検出 / 生物発光 / ルシフェリン / 核酸プローブ / 核酸化学 |
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| Outline of Final Research Achievements |
In this research, I focused on the to development of a novel gene detection system by utilizing "bioluminescence", which was unexplored in the field of gene detection technology. To achieve this goal, I carried out the following two studies. In the first study, I developed a system to convert sequence information into bioluminescence signal. I designed and chemically synthesized novel nucleic acid probe that releases a bioluminescent substrate upon binding to the target sequence. In the second study, I have developed the method for introducing guanidine nucleic acid, which possesses positive charges instead of negative charges, into oligonucleotide with the aim to strengthen the function of nucleic acid probe.
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Report
(4 results)
Research Products
(8 results)
