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Building up the bioluminescence molecule on the nucleic acid sequence and its application to gene detection technology

Research Project

Project/Area Number 15K14933
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Chemical pharmacy
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

KOJIMA Naoshi  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30356985)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords遺伝子検出 / 生物発光 / ルシフェリン / 核酸プローブ / 核酸化学
Outline of Final Research Achievements

In this research, I focused on the to development of a novel gene detection system by utilizing "bioluminescence", which was unexplored in the field of gene detection technology. To achieve this goal, I carried out the following two studies. In the first study, I developed a system to convert sequence information into bioluminescence signal. I designed and chemically synthesized novel nucleic acid probe that releases a bioluminescent substrate upon binding to the target sequence. In the second study, I have developed the method for introducing guanidine nucleic acid, which possesses positive charges instead of negative charges, into oligonucleotide with the aim to strengthen the function of nucleic acid probe.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] An alkylating immobilization linker for immunochemical epigenetic assessment2017

    • Author(s)
      Kurinomaru Takaaki、Kojima Naoshi、Kurita Ryoji
    • Journal Title

      Chemical Communications

      Volume: 53 Issue: 59 Pages: 8308-8311

    • DOI

      10.1039/c7cc02883k

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a 3’-amino linker with high conjugation activity and its application to covalently cross-link blunt ends of a duplex2016

    • Author(s)
      Keiko Kowata, Naoshi Kojima, Yasuo Komatsu
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 24 Issue: 9 Pages: 2108-2113

    • DOI

      10.1016/j.bmc.2016.03.039

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 抗がん剤を有する新規リンカー分子を用いたメチル化DNA免疫センサーの開発2017

    • Author(s)
      栗之丸隆章、小島 直、吉岡恭子、栗田僚二
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県横浜市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Immunochemical Sensing of Epigenomic Modification Using An Alkylating Immobilization Linker2017

    • Author(s)
      栗之丸隆章、小島 直、栗田僚二
    • Organizer
      第44回国際核酸化学シンポジウム(ISNAC2017、日本核酸科学会第1回年会)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 産業技術総合研究所ホームページ バイオメディカル研究部門 研究グループ紹介

    • URL

      https://unit.aist.go.jp/bmd/gr/nbd-4/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 産業技術総合研究所 バイオメディカル研究部門 研究グループ紹介 ナノバイオデバイス研究グループ

    • URL

      https://unit.aist.go.jp/bmd/gr/nbd-4/index.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 産業技術総合研究所ホームページ バイオメディカル研究部門 研究グループ紹介

    • URL

      https://unit.aist.go.jp/bmd/gr/nbd-4/index.html

    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 核酸の液中固定化剤2017

    • Inventor(s)
      栗田僚二、小島 直、栗之丸隆章
    • Industrial Property Rights Holder
      栗田僚二、小島 直、栗之丸隆章
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-033930
    • Filing Date
      2017-02-24
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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