Debelopment of nano satelite-organized archetecture as a technology to control an intracellular trafficking

• Project/Area Number: 15K14934
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Chiba University (2016); Hokkaido University (2015)
• Principal Investigator: Akita Hidetaka 千葉大学, 大学院薬学研究院, 教授 (80344472)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞内動態 / ナノ粒子 / ナノ材料 / 自己組織化

Outline of Final Research Achievements

For the development of efficient gene carrier, hierarchically ordered nano-architecture on which functional devices (ligand for specific tissues/cells/organelles) could be placed in desired manner was formulated. Environment sensitive lipid like material which we refer as to SS-cleavable Proton-Activated Lipid-like Material (ssPalm) was employed as a basis of nano-architecture. The hierarchical nano-architecture, Nano-size Satellite-type Organized Lipid-nanoparticle Assembly (Nano-SOLA), was fabricated via double strand formation of PEGylated complementary oligo DNA. The Nano-SOLA composed of multiple primary particles were recognized as one complex by the cells.

Research Products

• [Journal Article] Temperature and pH sensitivity of a stabilized self-nanoemulsion formed using an ionizable lipid-like material via an oil-to-surfactant transition. (2017)
  • Author(s): Tanaka H, Oasa S, Kinjo M, Tange K, Nakai Y, Harashima H, Akita H
  • Journal Title: Colloids Surf B Biointerfaces Volume: 151 Pages: 95-101
  • DOI: 10.1016/j.colsurfb.2016.11.020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cellular environment-responsive nanomaterials for use in gene and siRNA delivery: molecular design for biomembrane destabilization and intracellular collapse (2016)
  • Author(s): Tanaka H, Sato Y, Harashima H, Akita H
  • Journal Title: Expert Opin Drug Deliv. Volume: 13(7) Issue: 7 Pages: 1015-1027
  • DOI: 10.1517/17425247.2016.1154531
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of particle size on their accumulation in an inflammatory lesion in a dextran sulfate sodium (DSS)-induced colitis model. (2016)
  • Author(s): Watanabe A, Tanaka H, Sakurai Y, Tange K, Nakai Y, Ohkawara T, Takeda H, Harashima H, Akita H.
  • Journal Title: Int J Pharm Volume: 509(1-2) Issue: 1-2 Pages: 118-122
  • DOI: 10.1016/j.ijpharm.2016.05.043
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cellular environment-responsive nanomaterials for use in gene and siRNA delivery: molecular design for biomembrane destabilization and intracellular collapse. (2016)
  • Author(s): Tanaka H, Sato Y, Harashima H, Akita H.
  • Journal Title: Expert Opin Drug Deliv. Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular tuning of a vitamin E-scaffold pH-sensitive and reductive cleavable lipid-like material for accelerated in vivo hepatic siRNA delivery (2015)
  • Author(s): Akita, H. Noguchi Y, Hatakeyama H, Tange K, Nakai Y, Harashima H
  • Journal Title: ACS Biomater Sci Eng. Volume: 1 Issue: 9 Pages: 834-844
  • DOI: 10.1021/acsbiomaterials.5b00203
  • Peer Reviewed / Open Access
• [Presentation] 体内・細胞内動態の情報に基づくベクター開発と蛍光技術を用いたナノ粒子物性評価 (2016)
  • Author(s): 秋田 英万
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-27
  • Invited
• [Presentation] Particle formed with ssPalm as a multi-nanoDDS platform (2016)
  • Author(s): Hidetaka Akita
  • Organizer: 3rd International Conference on Biomaterials Science in Tokyo (ICBS2016)
  • Place of Presentation: 東京大学本郷キャンパス
  • Int'l Joint Research / Invited
• [Presentation] 細胞内環境応答性脂質様材料を基盤としたマルチ創剤基盤 (2016)
  • Author(s): 秋田 英万
  • Organizer: 第31回 日本薬物動態学会
  • Place of Presentation: 松本 キッセイ文化ホール
  • Int'l Joint Research / Invited
• [Presentation] Development of a nanoDDS platform based on an analysis of intracellular trafficking (2015)
  • Author(s): 秋田 英万
  • Organizer: 日本薬物動態学会 第30回年会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-11-12
  • Invited
• [Presentation] Functionalized Self-Nanoemulsion formed with SS-cleavable and pH-activated Lipid-like Material via Oil-to-Surfactant Transition (2015)
  • Author(s): Hiroki Tanaka, Syo Oasa, Masataka Kinjo, Kota Tange, Yuta Nakai, and Hideyoshi Harashima, Hidetaka Akita
  • Organizer: 第9回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 千葉大学（千葉県千葉市）
  • Year and Date: 2015-11-07
• [Presentation] INVESTIGATION OF OPTIMAL NANOPARTICLE PROPERTIES ON DELIVERY TO THE INFLAMED COLON (2015)
  • Author(s): Ayaka Watanabe, Hiroki Tanaka, Tatsuya Ohkawara, Kota Tange, Hiroshi Takeda, Hideyoshi Harashima, Hidetaka Akita
  • Organizer: 第9回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 千葉大学（千葉県千葉市）
  • Year and Date: 2015-11-07
• [Patent(Industrial Property Rights)] Ｏ／Ｗ型エマルション (2015)
  • Inventor(s): 原島秀吉、秋田英万、田中浩揮、渡邊綾香など７名
  • Industrial Property Rights Holder: 北海道大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-200148
  • Filing Date: 2015-10-08