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Cell surface modification using non-natural amino acid and its application to drug delivery system

Research Project

Project/Area Number 15K14940
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Physical pharmacy
Research InstitutionKyoto University

Principal Investigator

Hashida Mitsuru  京都大学, 薬学研究科, 教授 (20135594)

Co-Investigator(Kenkyū-buntansha) 樋口 ゆり子  京都大学, 薬学研究科, 講師 (40402797)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞膜修飾 / 非天然アミノ酸 / VHH / 細胞接着 / 細胞製剤 / DDS / 細胞治療 / ドラッグデリバリー
Outline of Final Research Achievements

The purpose of this study is to enhance the cell-cell adhesion by introducing the protein ligand via PEG-lipid to cells. Variable domain of heavy chain of heavy chain antibody (VHH) against mCherry was used as a model protein ligand. Azide group was introduced into VHH at the selected position as the connection of PEG-lipid through azide group does not disturb antigen binding. VHHs were modified on the cell surface by adding Az-VHH after cells were coated with DBCO-PEG-DSPE. VHH modified on the cell surface could specifically bind with antigen. Adhesion to antigen-coated plate was enhanced by VHH-modification to cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Presentation (3 results)

  • [Presentation] mKO2の特定の位置で結合したmKO2-PEG-DSPE結合体の合成と細胞膜表面修飾への応用2016

    • Author(s)
      ○樋口ゆり子、杉田かおり、山下富義、橋田 充
    • Organizer
      日本薬剤学会第31年会
    • Place of Presentation
      長良川国際会議場(岐阜)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 非天然アミノ酸を介して結合させたタンパク質-PEG脂質誘導体による細胞膜表面修飾2015

    • Author(s)
      ○杉田かおり、樋口ゆり子、山下富義、橋田 充
    • Organizer
      第9回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      千葉大学薬学研究院 亥鼻キャンパス(千葉県)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] ペプチドPEG脂質を用いて表面修飾された間葉系幹細胞と血管内皮細胞との接着における鎖長の影響2015

    • Author(s)
      ○樋口ゆり子、小田敬昌、山下富義、橋田 充
    • Organizer
      第31回日本DDS学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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