Study on rapid and hypersensitive screening of chemical genotoxin using mammalian cells defective for DNA damage-response factors
| Project/Area Number |
15K14953
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Gakushuin University |
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Principal Investigator |
HANAOKA Fumio 学習院大学, 理学部, 研究員 (50012670)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ゲノム / DNA損傷 / 損傷乗り越え合成 / 損傷応答 / 遺伝毒性 / スクリーニング系 / マウス胚性線維芽細胞 / DNAポリメラーゼ / DNA修復 |
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| Outline of Final Research Achievements |
In order to establish a rapid, simple and hypersensitive method of screening various genotoxic compounds for mammalian cells, we compared sensitivities to various compounds among mouse embryonic fibroblasts derived from wild-type (WT) and knock-out mice lacking one of three Y-family TLS DNA polymerases (Pol eta, Pol iota, and Pol kappa) or all of them (TKO). We found that TKO cells exhibited the highest sensitivities to most of the tested genotoxins, but not to the non-genotoxins. Furthermore, we found that ratios of half-maximal inhibitory concentration of WT and TKO cells would be nice markers to quantitatively evaluate the hypersensitivity of TKO cells to different chemicals.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Direct single-molecule observations of DNA unwinding by SV40 large tumor antigen under a negative DNA supercoil state.2018
Author(s)
Takahashi S, Motooka S, Kawasaki S, Kurita H, Mizuno T, Matsuura SI, Hanaoka F, Mizuno A, Oshige M, Katsura S
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Journal Title
Journal of Biomolecular Structure and Dynamics,
Volume: 5
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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