| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The potential of filamentous fungi as a rich source of natural products has been unveiled by genome sequencing studies that have shown the presence of many cryptic biosynthetic gene clusters. The access for secondary metabolites hidden in the cryptic biosynthetic pathway would increase the opportunity to discover new drug candidates. In this study, to develop a new method to stimulate cryptic biosynthetic pathway in fungi, we introduced “ribosome engineering of bacteria” into fungal secondary metabolism.
We obtained mutant strains with hygromycin B resistance from various fungi by mutagenetic approach using MNNG as a chemical mutagen. The evaluation of the secondary metabolite productivities of the mutant strains indicated that introduction of hygromycin B resistance into fungi is available to activate fungal metabolite productions. Application of the method to Chaetomium and Cordyceps fungi allowed us to isolate novel compounds whose production were silent in their wild type strains.
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