Creation of novel DNA ligands with potent analgesic action against MOR opioid receptor and elucidation of molecular mechanisms.
| Project/Area Number |
15K14985
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
IKEMOTO Mitsushi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50356424)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAGISHI Makoto 国立研究開発法人・産業技術総合研究所, バイオメディカル研究部門, グループ長 (30323538)
ISHII Noriyuki 国立研究開発法人・産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (10261174)
YAMAZAKI Kazuhiko 国立研究開発法人・産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00358243)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | アプタマー / DNA / GPCRs / オピオイド受容体 / 探索方法 / 鎮痛 / 創薬基盤技術 / Cell SELEX法 / DNAアプタマー / G蛋白質受容体 / 新規核酸リガンド探索法 / 膜小胞 / 鎮痛効果 / 新規核酸アプタマー探索法 / モルヒネ |
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| Outline of Final Research Achievements |
We have established a novel DNA aptamer screening method utilizing the physiological functions of GPCRs to develop DNA aptamers targeting G protein coupled receptors (GPCRs). As a result of many studies, this method was more sensitive and efficient than Cell SELEX method. As a result of a search using this screening technology, we obtained a DNA aptamer, designated Apt-MOR, which recognized MOR opioid receptor, an analgesic-related GPCR. This DNA aptamer bound specifically to MOR with high affinity and functioned as an agonist for MOR. Therefore, this novel screening method and Apt-MOR DNA aptamer can be available tools for developing analgesic drug discovery technology.
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Report
(3 results)
Research Products
(6 results)
