Development of cell sensor by using RNA binding protein

Research Project

Project/Area Number	15K14987
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Environmental and hygienic pharmacy
Research Institution	The University of Tokyo
Principal Investigator	Akimitsu Nobuyoshi 東京大学, アイソトープ総合センター, 教授 (40294962)
Research Collaborator	YAMADA Toshimichi 東京大学, アイソトープ総合センター, 特任研究員
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	RNA / 細胞センサー / ノンコーディングRNA / RNA結合タンパク質
Outline of Final Research Achievements	In this study, I design PUM, an RNA binding protein, conjugated Green Fluorescent Protein (GFP) to establish the cell line for monitoring RNA expressions in vivo. To this end, at first, I determined the RNAs that are controlled by PUM in vivo. By integrating RIP-seq data and BRIC-seq data, I determined 50 PUM targets. Based on this data, I designed PUM-GFP construct and established the cells expressing PUM-GFP.