| Project/Area Number |
15K14987
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
YAMADA Toshimichi 東京大学, アイソトープ総合センター, 特任研究員
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | RNA / 細胞センサー / ノンコーディングRNA / RNA結合タンパク質 |
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| Outline of Final Research Achievements |
In this study, I design PUM, an RNA binding protein, conjugated Green Fluorescent Protein (GFP) to establish the cell line for monitoring RNA expressions in vivo. To this end, at first, I determined the RNAs that are controlled by PUM in vivo. By integrating RIP-seq data and BRIC-seq data, I determined 50 PUM targets. Based on this data, I designed PUM-GFP construct and established the cells expressing PUM-GFP.
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