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Genomics-based improvement of Vero cells for virus growth

Research Project

Project/Area Number 15K14993
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental and hygienic pharmacy
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Hanada Kentaro  国立感染症研究所, 細胞化学部, 部長 (30192701)

Co-Investigator(Kenkyū-buntansha) 齊藤 恭子  国立感染症研究所, 細胞化学部, 主任研究官 (70235034)
Co-Investigator(Renkei-kenkyūsha) YAMAJI Toshiyuki  国立感染症研究所, 細胞化学部, 室長 (50332309)
FUKASAWA Masayoshi  国立感染症研究所, 細胞化学部, 室長 (20291130)
SAKUMA Chisato  国立感染症研究所, 細胞化学部, 研究員 (80782888)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞基材 / ベロ細胞 / 宿主細胞 / ゲノム編集 / ウイルス / ワクチン / 感染症 / フラビウイルス / ウイルス感染症 / Vero細胞 / Huh細胞
Outline of Final Research Achievements

We recently examined the whole genome sequence of African green monkey kidney-derived Vero cells susceptible to various virus types. We herein prepared basic experimental conditions to produce new Vero cell variants that support better virus proliferation using genome editing technology. The following results were obtained. (1) Experimental conditions to isolate a Vero cell clone sustaining high susceptibility to Japanese encephalitis virus (JEV) were established. (2) The genome-wide screening of genes, the disruption of which confers Vero cells with resistance to Shiga-like toxin, was conducted. This screening hit many genes involved in the biosynthesis of the toxin receptor glycosphingolipid Gb3, which confirmed that the human CRISPR single-guide RNA library is applicable to Vero cells. (3) Among four Vero sublines, a parental Vero cell subline suitable for a somatic genetic approach was selected based on the stability of its karyotype and the sustainability of JEV susceptibility.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (4 results)

  • [Journal Article] Meeting report: The first meeting of the national control laboratories for vaccines and biologicals in the Western pacific in 20162016

    • Author(s)
      Hokyung Oh, Jinho Shin, Manabu Ato, Xiao Ma, David Williams, Kiwon Han, Yang Jin Kim, Hyung Goo Kang, Kikyung Jung, Kentaro Hanada, Masaki Ochiai, Pham Van Hug, Sangmi Park, and Chiyoung Ahn
    • Journal Title

      Osong Public Health and Research Perspectives

      Volume: 8 Issue: 1 Pages: 91-103

    • DOI

      10.24171/j.phrp.2017.8.1.13

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] カルタヘナ法と関わる組換え生物生ワクチン2017

    • Author(s)
      花田賢太郎
    • Organizer
      バイオロジクスフォーラム第14回学術集会
    • Place of Presentation
      東京
    • Related Report
      2016 Annual Research Report
  • [Presentation] Vero cells: Determination of the genome landscape and its application to QC2016

    • Author(s)
      Kentaro Hanada
    • Organizer
      Workshop of Western Pacific National Control Laboratories for Vaccines and Blood Products
    • Place of Presentation
      韓国ソウル市
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Vero細胞基材の品質管理に資する基盤研究2016

    • Author(s)
      花田賢太郎
    • Organizer
      バイオロジクスフォーラム第13回学術集会
    • Place of Presentation
      東京
    • Year and Date
      2016-02-29
    • Related Report
      2015 Research-status Report
    • Invited
  • [Remarks] Vero細胞の物語 ~その樹立からゲノム構造の決定、そして未来へ~

    • URL

      http://www.niid.go.jp/niid/ja/chlamydia-pneumonia-m/818-biochem/5752-vero.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 動物細胞培養に関する用語など

    • URL

      http://www.niid.go.jp/niid/ja/chlamydia-std-m/1880-biochem/3255-2013-02-25-05-58-54.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] Vero細胞の物語 ~その樹立からゲノム構造の決定、そして未来へ~

    • URL

      http://www.nih.go.jp/niid/ja/chlamydia-pneumonia-m/818-biochem/5752-vero.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 動物細胞培養に関する用語など

    • URL

      http://www.nih.go.jp/niid/ja/chlamydia-std-m/1880-biochem/3255-2013-02-25-05-58-54.html

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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