Development of a comprehensive drug transporter identification method by gene knockout

Research Project

Project/Area Number	15K14998
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Medical pharmacy
Research Institution	The University of Tokyo
Principal Investigator	Kusuhara Hiroyuki 東京大学, 大学院薬学系研究科(薬学部), 教授 (00302612)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	トランスポーター / CRISPR-Cas9システム / 発現抑制 / オーファントランスポーター / CRISPR-Cas9 / 遺伝子ノックアウト / ほ乳類細胞 / CRISPR Cas9システム / ライブラリー / レンチウィルス
Outline of Final Research Achievements	The aim of this research was to establish inhibition of transporter gene expression by CRISPR-Cas9 system in mammalian cells as transporter identification tool. We designed guide RNA for all human SLC transporters and succeeded in simultaneous knockout of multiple transporters. On the other hand, we failed to suppress expression of some genes in the host cells after infection of viruses. Improvements in the design method of guide RNA are future subjects.

Report

(3 results)