Development of a comprehensive drug transporter identification method by gene knockout
| Project/Area Number |
15K14998
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kusuhara Hiroyuki 東京大学, 大学院薬学系研究科(薬学部), 教授 (00302612)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | トランスポーター / CRISPR-Cas9システム / 発現抑制 / オーファントランスポーター / CRISPR-Cas9 / 遺伝子ノックアウト / ほ乳類細胞 / CRISPR Cas9システム / ライブラリー / レンチウィルス |
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| Outline of Final Research Achievements |
The aim of this research was to establish inhibition of transporter gene expression by CRISPR-Cas9 system in mammalian cells as transporter identification tool. We designed guide RNA for all human SLC transporters and succeeded in simultaneous knockout of multiple transporters. On the other hand, we failed to suppress expression of some genes in the host cells after infection of viruses. Improvements in the design method of guide RNA are future subjects.
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Report
(3 results)
Research Products
(1 results)
