Mechanism of postprandial hyperglycemia due to transporter gene knockout and identification of endogenous substrates

• Project/Area Number: 15K15000
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Kanazawa University
• Principal Investigator: KATO YUKIO 金沢大学, 薬学系, 教授 (30251440)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Yasuhiko 金沢大学, 医学系, 教授 (20313637) ; KITAMURA Masanori 金沢大学, 薬学系, 准教授 (80453835) ; NAKAMICHI Noritaka 金沢大学, 薬学系, 准教授 (10401895)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膜輸送体 / ブドウ糖 / ミトコンドリア / メタボローム / 糖代謝

Outline of Final Research Achievements

We have previously established gene knockout mice for the solute carrier membrane transporter which is expressed in ubiquitous organs and mainly accepts organic cations as substrates. Our previous metabolome analyses using both wild-type and knockout mice have suggested possible change in glucose metabolism in the body due to the gene knockout. Identification of the molecular mechanism may lead to clarifying the regulatory mechanism for glucose homeostasis and diabetics. Therefore, the aim of the present study is to clarify the role of thie transporter in glucose metabolism and to identify its endogenous substrates.

Research Products

• [Journal Article] トランスポーター介在性ブレインフードとしてのエルゴチオネイン (2017)
  • Author(s): 加藤将夫、三田村萌恵、松本 聡
  • Journal Title: FOOD Style Volume: 21
  • NAID: 40021087478
• [Journal Article] Preventive effect of salicylate and pyridoxamine on diabetic nephropathy (2016)
  • Author(s): Abouzed TK, Munesue S, Harashima A, Masuo Y, Kato Y, Kahilo KK, Yamamoto H, Yamamoto Y
  • Journal Title: J. Diabetes Res Volume: 1786789 Pages: 1-10
  • DOI: 10.1155/2016/1786789
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dipeptide species regulate nutrient signalling essential for the maintenance of chronic myelogenous leukaemia stem cells. (2015)
  • Author(s): Naka K, Jomen Y, Ishihara K, Kim J, Ishimoto T, Bae E, Mohney R, Stirdivant SM, Oshima H, Oshima M, Kim DW, Nakauchi H, Takihara Y, Kato Y, Ooshima A, Kim SJ.
  • Journal Title: Nature Communication Volume: 6 Issue: 1 Pages: 8039-8039
  • DOI: 10.1038/ncomms9039
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Role of ABC xenobiotic transporters in drug disposition in the skin. (2015)
  • Author(s): Kato Y, Masuo Y and Nakamichi N.
  • Organizer: “The Best of the Rest: Drug Transport across Non-traditional Biological Barriers,” 2015 AAPS Annual Meeting and Exposition
  • Place of Presentation: Orange Country Convention Center, Orlando
  • Year and Date: 2015-10-25
  • Int'l Joint Research / Invited
• [Presentation] ABC transporters involved in xenobiotics disposition in the skin. (2015)
  • Author(s): Kato Y, Masuo Y and Nakamichi N.
  • Organizer: Meet the Experts: Transporter Conference Tokyo 2015
  • Place of Presentation: Tokyo Marriott Hotel
  • Year and Date: 2015-05-13
  • Int'l Joint Research / Invited
• [Remarks] 金沢大学分子薬物治療学研究室
  • URL: http://www.p.kanazawa-u.ac.jp/~bunyaku/