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Measurements of cellular mechanics using super resolution microscope

Research Project

Project/Area Number 15K15010
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionHokkaido University

Principal Investigator

MIZUTANI TAKEOMI  北海道大学, 先端生命科学研究院, 助教 (40451405)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞運動 / 力学 / 共同現象 / 生物物理学 / メカノセンス / 細胞協調
Outline of Final Research Achievements

During the generation of organs, cells should migrate not in a random walk but in a directional mode (collective migration). In this project, we measured mechanical and biochemical information within collective cell migration, and revealed an aspect of the mechanism of collective migration. As a result, we found that cell-cell junction is essential for the collective migration.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 5 results,  Open Access: 5 results) Presentation (2 results) Book (1 results) Remarks (2 results)

  • [Journal Article] High-Resolution Vertical Observation of Intracellular Structure using Magnetically Responsive Microplates2016

    • Author(s)
      Tetsuhiko Teshima, Hiroaki Onoe, Soichiro Tottori, Hiroka Aonuma, Takeomi Mizutani, Koki Kamiya, Hirotaka Ishihara, Hirotaka Kanuka, Shoji Takeuchi
    • Journal Title

      Small

      Volume: 12 Issue: 25 Pages: 3366-3373

    • DOI

      10.1002/smll.201600339

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Role of ATF5 in the invasive potential of diverse human cancer cell lines2016

    • Author(s)
      Akihiro Nukuda, Hiroki Endoh, Motoaki Yasuda, Takeomi Mizutani, Kazushige Kawabata, Hisashi Haga
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 474(3) Issue: 3 Pages: 509-514

    • DOI

      10.1016/j.bbrc.2016.04.131

    • NAID

      120006219898

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Heterogeneous Filament Network Formation by Myosin Light Chain Isoforms Effects on Contractile Energy Output of Single Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells2016

    • Author(s)
      Takeomi Mizutani, Kazuya Kurusawa, Hisashi Haga, Kazushige Kawabata
    • Journal Title

      Regenerative Therapy

      Volume: 3 Pages: 90-96

    • DOI

      10.1016/j.reth.2016.02.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells2016

    • Author(s)
      Takeomi Mizutani, Hisashi Haga, Kazushige Kawabata
    • Journal Title

      Data in Brief

      Volume: 6 Pages: 793-798

    • DOI

      10.1016/j.dib.2015.12.053

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transgene Integration into the Human AAVS1 Locus Enhances Myosin II-Dependent Contractile Force by Reducing Expression of Myosin Binding Subunit 852015

    • Author(s)
      T. Mizutani, R. Li, H. Haga, and K. Kawabata
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 465 Issue: 2 Pages: 270-274

    • DOI

      10.1016/j.bbrc.2015.08.018

    • NAID

      120005906854

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Three-dimensional morphogenesis of MDCK cells induced by cellular contractile forces on a viscous substrate2015

    • Author(s)
      Misako Imai, Kazuya Furusawa, Takeomi Mizutani, Kazushige Kawabata, Hisashi Haga
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 14208-14208

    • DOI

      10.1038/srep14208

    • NAID

      120005682122

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 細胞、多細胞集団、個体での細胞の運動と力学量制御2016

    • Author(s)
      水谷 武臣、川端 和重
    • Organizer
      第27回バイオフロンティア講演会
    • Place of Presentation
      北海道大学 (北海道・札幌市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞の力学計測と応用展開2015

    • Author(s)
      水谷 武臣、李 瑞、芳賀 永、川端 和重
    • Organizer
      細胞運動研究会
    • Place of Presentation
      北海道大学(北海道・札幌市)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report
  • [Book] 「3次元化細胞の力学」 細胞社会学2016

    • Author(s)
      水谷 武臣
    • Total Pages
      13
    • Publisher
      コロナ社
    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      http://altair.sci.hokudai.ac.jp/g3/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究室のHP

    • URL

      http://altair.sci.hokudai.ac.jp/g3/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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