| Project/Area Number |
15K15012
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
水谷 祐輔 新潟大学, 医歯学系, 助教 (40646238)
中島 真人 新潟大学, 医歯学総合病院, 助教 (60588250)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 走査電子顕微鏡 / 細胞と組織 / ライブイメージング / 大気圧 / バイオイメージング / 大気圧観察 / 細胞 / 組織 / 生きた観察 |
|---|
| Outline of Final Research Achievements |
We investigated the possibility of observing live cells and tissues by atmospheric scanning electron microscopy (atmospheric SEM). Our study revealed that this technique is powerful for observing plant tissues including leaf surfaces, but has some difficulty for visualizing the surface of animal cells and tissues due to several reasons such as surface unevenness, low signal intensity and the presence of surface water . To improve the quality of cell observation by atmospheric SEM, preparation techniques including the ion liquid treatment were tested in this study.
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