| Project/Area Number |
15K15018
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大野 芳典 広島大学, 原爆放射線医科学研究所, 助教 (10548986)
安永 晋一郎 福岡大学, 医学部, 教授 (50336111)
白井 学 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (70294121)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 心筋 / 細胞周期 / 幹細胞 / ポリコーム遺伝子群 / Geminin / 心筋分裂 / 心臓幹細胞 / ポリコーム複合体 |
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| Outline of Final Research Achievements |
Polycomb-group (PcG) genes play an important role in cardiac development as well as in maintenance of cardiac muscle. Molecularly PcG genes regulate gene transcription through an epigenetic mechanism and also act as an E3 ubiquitin ligase for Geminin, which regulates cellular proliferation and differentiation. In this study we generated Geminin-EYFP knock-in mice to directly trace Geminin expression in vivo and detected a candidate for cell-cycling cardiac muscle or cardiac stem cells. We also generated cell-penetrating Geminin, which is transduced directly into cells, to manipulate expression levels of Geminin protein in cells. We anticipate that these technologies are helpful for analyzing cell cycling of cardiac muscle and also function of cardiac stem cells.
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