| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We succeeded in immortalization of reporter mouse mammary epithelial cells (MECs), which allow us to screen genes involved in dedifferentiation, efficiently. 115 genes, which had been first under expectation as associated genes with dedifferentiation, unfortunately did not activate reporter cells. Thus, there was a need to evaluate larger gene sets. Then we developed a gene trap system for expression of genes covering more broad set including non-coding RNA using a transposon system. This is powerful technique one because there is no need to prepare cDNA libraries. Using this convenient and efficient system, we isolated 4 positive clones. Among them, we identified a candidate gene possibly involving regulation of stemness, and now are analyzing its function both in vitro and in vivo. Moreover, by applying the technique of the trap method, various technologies for evaluating the function of genes regulating stemness were also developed.
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