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Screening for genes involved in efficient reprogramming directly into mammary stem cells

Research Project

Project/Area Number 15K15022
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionWaseda University

Principal Investigator

Semba Kentaro  早稲田大学, 理工学術院, 教授 (70206663)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords細胞分化 / 組織形成 / 乳腺 / 幹細胞 / ダイレクトリプログラミング / 癌幹細胞 / リプログラミング / 遺伝子スクリーニング / トラップ法 / 乳腺組織 / 脱分化
Outline of Final Research Achievements

We succeeded in immortalization of reporter mouse mammary epithelial cells (MECs), which allow us to screen genes involved in dedifferentiation, efficiently. 115 genes, which had been first under expectation as associated genes with dedifferentiation, unfortunately did not activate reporter cells. Thus, there was a need to evaluate larger gene sets. Then we developed a gene trap system for expression of genes covering more broad set including non-coding RNA using a transposon system. This is powerful technique one because there is no need to prepare cDNA libraries. Using this convenient and efficient system, we isolated 4 positive clones. Among them, we identified a candidate gene possibly involving regulation of stemness, and now are analyzing its function both in vitro and in vivo. Moreover, by applying the technique of the trap method, various technologies for evaluating the function of genes regulating stemness were also developed.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016

All Presentation (8 results)

  • [Presentation] 高感度トランスポゾントラップベクターを用いた遺伝子発現応答細胞の単離2017

    • Author(s)
      石川公輔
    • Organizer
      日本がん分子標的治療学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高感度トランスポゾントラップベクターを用いた遺伝子発現応答細胞の単離2017

    • Author(s)
      若林佑太郎
    • Organizer
      日本がん分子標的治療学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高感度トラップベクターを用いたレポーター細胞の作製2017

    • Author(s)
      石川公輔
    • Organizer
      日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 乳がん遺伝子解析のためのマウス in vivo発現系の開発2017

    • Author(s)
      多ヶ谷紘壮
    • Organizer
      日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 乳腺細胞系譜特異的な遺伝子発現ベクターの開発2017

    • Author(s)
      上岡有紀乃
    • Organizer
      金沢大学がん進展制御研究所共同利用・共同研究拠点シンポジウム
    • Place of Presentation
      金沢東急ホテル
    • Related Report
      2016 Research-status Report
  • [Presentation] Development of highly sensitive promoter-trap vector system2016

    • Author(s)
      石川公輔
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Research-status Report
  • [Presentation] 高感度トランスポゾントラップベクターを用いた刺激応答細胞と遺伝子の単離技術の開発2016

    • Author(s)
      小林雄太
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Research-status Report
  • [Presentation] BACベクターを用いた遺伝子導入乳腺再構築技術2016

    • Author(s)
      上岡有紀乃
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-12-27  

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