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Verification of genomic diversity required for tumorigenesis

Research Project

Project/Area Number 15K15024
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionTohoku University

Principal Investigator

NAKAYAMA KEIKO  東北大学, 医学(系)研究科(研究院), 教授 (60294972)

Co-Investigator(Kenkyū-buntansha) FUNAYAMA RYO  東北大学, 大学院医学系研究科, 助教 (20452295)
NAKAGAWA TADASHI  東北大学, 大学院医学系研究科, 助教 (30707013)
HOSOGANE MASAKI  東北大学, 大学院医学系研究科, 助手 (30734347)
Project Period (FY) 2015-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords腫瘍形成 / CRISPR/Cas9 / 次世代シークエンサー / 乳癌細胞株 / 免疫不全マウス
Outline of Final Research Achievements

It has been demonstrated that solid tumors are composed of various genomic mutated cells by genomic sequencing and analysis of diversity. In this program, we tried to identify critical mutations to generate solid tumors by random mutagenesis using CRISPR/Cas9 library. We used library which randomly made deletions at promoter regions of almost all reported human genes. We infected CRISPR/Cas9 library to human cancer cell lines to generate randomly mutated cell pool. Diversity of library extracted cell line were reduced within several days culture after introduction of library compared to that of original library. This observation suggest that in introduction of library make some clones die or proliferate less, as these clones have disadvantage to survival in vitro compared to other clones.

Report

(2 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (11 results)

All 2015 Other

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Genomic analysis identifies candidate pathogenic variants in 9 of 18 patients with unexplained West syndrome.2015

    • Author(s)
      Hino-Fukuyo N, Kikuchi A, Arai-Ichinoi N, Niihori T, Sato R, Suzuki T, Kudo H, Sato Y, Nakayama T, Kakisaka Y, Kubota Y, Kobayashi T, Funayama R, Nakayama K, Uematsu M, Aoki Y, Haginoya K, Kure S.
    • Journal Title

      Hum Genet.

      Volume: 134 Issue: 6 Pages: 649-658

    • DOI

      10.1007/s00439-015-1553-6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mutations in PIGL in a patient with Mabry syndrome.2015

    • Author(s)
      Fujiwara I, Murakami Y, Niihori T, Kanno J, Hakoda A, Sakamoto O, Okamoto N, Funayama R, Nagashima T, Nakayama K, Kinoshita T, Kure S, Matsubara Y, Aoki Y.
    • Journal Title

      Am J Med Genet A.

      Volume: in press Issue: 4 Pages: 777-785

    • DOI

      10.1002/ajmg.a.36987

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Protein monoubiquitylation: targets and diverse functions2015

    • Author(s)
      Nakagawa T, Nakayama K
    • Journal Title

      Genes to cells

      Volume: 7 Issue: 7 Pages: 543-562

    • DOI

      10.1111/gtc.12250

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of the Atg9a gene in intrauterine growth and survival of fetal mice.2015

    • Author(s)
      Kojima T, Yamada T, Akaishi R, Furuta I, Saitoh T, Nakabayashi K, Nakayama KI, Nakayama K, Akira S, Minakami H
    • Journal Title

      Reprod.Biol.

      Volume: 15 Issue: 3 Pages: 131-138

    • DOI

      10.1016/j.repbio.2015.05.001

    • NAID

      120005828503

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The artificial loss of Runx1 reduces the expression of quiescence-associated transcription factors in CD4(+) T lymphocytes.2015

    • Author(s)
      Wong WF, Kohu K, Nagashima T, Funayama R, Matsumoto M, Movahed E, Tan GM, Yeow TC, Looi CY, Kurokawa M, Osato M, Igarashi K, Nakayama K, Satake M.
    • Journal Title

      Mol Immunol.

      Volume: 68 Issue: 2 Pages: 223-33

    • DOI

      10.1016/j.molimm.2015.08.012

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rho-kinase inhibition during early cardiac development causes arrhythmogenic right ventricular cardiomyopathy in mice2015

    • Author(s)
      Ellawindy A, Satoh K, Sunamura S, Kikuchi N, Suzuki K, Minami T, Ikeda S, Tanaka S, Shimizu T, Enkhjargal B, Miyata S, Taguchi Y, Handoh T, Kobayashi K, Kobayashi K, Nakayama K, Miura M, Shimokawa H.
    • Journal Title

      Arterioscler Thromb Vasc Biol.

      Volume: 35 Issue: 10 Pages: 2172

    • DOI

      10.1161/atvbaha.115.305872

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] S6 Kinase- and beta-TrCP2-Dependent Degradation of p19Arf Is Required for Cell Proliferation2015

    • Author(s)
      Nakagawa T, Araki T, Nakagawa M, Hirao A, Unno M, Nakayama K
    • Journal Title

      Molecular and cellular biology

      Volume: 20 Issue: 20 Pages: 3517-3527

    • DOI

      10.1128/mcb.00343-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] セルトリ細胞特異的β-TrCPノックアウトマウスの解析2015

    • Author(s)
      諸星茜、中川直、中野星児、中山啓子
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] TGF-β刺激によるTFIID構成因子TAF7の分解とその役割の解明2015

    • Author(s)
      中川直、細金正樹、舟山亮、中山啓子
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] がん原遺伝子RASによる遺伝子サイレンシングの分子機構の解析2015

    • Author(s)
      舟山亮、細金正樹、長嶋剛史、中山啓子
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Remarks] がん医学コアセンター 細胞増殖制御分野

    • URL

      http://www.devgen.med.tohoku.ac.jp/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2017-05-10  

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