Verification of genomic diversity required for tumorigenesis
| Project/Area Number |
15K15024
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Tohoku University |
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Principal Investigator |
NAKAYAMA KEIKO 東北大学, 医学(系)研究科(研究院), 教授 (60294972)
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| Co-Investigator(Kenkyū-buntansha) |
FUNAYAMA RYO 東北大学, 大学院医学系研究科, 助教 (20452295)
NAKAGAWA TADASHI 東北大学, 大学院医学系研究科, 助教 (30707013)
HOSOGANE MASAKI 東北大学, 大学院医学系研究科, 助手 (30734347)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 腫瘍形成 / CRISPR/Cas9 / 次世代シークエンサー / 乳癌細胞株 / 免疫不全マウス |
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| Outline of Final Research Achievements |
It has been demonstrated that solid tumors are composed of various genomic mutated cells by genomic sequencing and analysis of diversity. In this program, we tried to identify critical mutations to generate solid tumors by random mutagenesis using CRISPR/Cas9 library. We used library which randomly made deletions at promoter regions of almost all reported human genes. We infected CRISPR/Cas9 library to human cancer cell lines to generate randomly mutated cell pool. Diversity of library extracted cell line were reduced within several days culture after introduction of library compared to that of original library. This observation suggest that in introduction of library make some clones die or proliferate less, as these clones have disadvantage to survival in vitro compared to other clones.
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Report
(2 results)
Research Products
(11 results)
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[Journal Article] Genomic analysis identifies candidate pathogenic variants in 9 of 18 patients with unexplained West syndrome.2015
Author(s)
Hino-Fukuyo N, Kikuchi A, Arai-Ichinoi N, Niihori T, Sato R, Suzuki T, Kudo H, Sato Y, Nakayama T, Kakisaka Y, Kubota Y, Kobayashi T, Funayama R, Nakayama K, Uematsu M, Aoki Y, Haginoya K, Kure S.
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Journal Title
Hum Genet.
Volume: 134
Issue: 6
Pages: 649-658
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mutations in PIGL in a patient with Mabry syndrome.2015
Author(s)
Fujiwara I, Murakami Y, Niihori T, Kanno J, Hakoda A, Sakamoto O, Okamoto N, Funayama R, Nagashima T, Nakayama K, Kinoshita T, Kure S, Matsubara Y, Aoki Y.
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Journal Title
Am J Med Genet A.
Volume: in press
Issue: 4
Pages: 777-785
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The artificial loss of Runx1 reduces the expression of quiescence-associated transcription factors in CD4(+) T lymphocytes.2015
Author(s)
Wong WF, Kohu K, Nagashima T, Funayama R, Matsumoto M, Movahed E, Tan GM, Yeow TC, Looi CY, Kurokawa M, Osato M, Igarashi K, Nakayama K, Satake M.
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Journal Title
Mol Immunol.
Volume: 68
Issue: 2
Pages: 223-33
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Rho-kinase inhibition during early cardiac development causes arrhythmogenic right ventricular cardiomyopathy in mice.2015
Author(s)
Ellawindy A, Satoh K, Sunamura S, Kikuchi N, Suzuki K, Minami T, Ikeda S, Tanaka S, Shimizu T, Enkhjargal B, Miyata S, Taguchi Y, Handoh T, Kobayashi K, Kobayashi K, Nakayama K, Miura M, Shimokawa H.
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Journal Title
Arterioscler Thromb Vasc Biol.
Volume: 35
Issue: 10
Pages: 2172-2184
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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