Development of IP3 clamp method
| Project/Area Number |
15K15027
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | The University of Electro-Communications |
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Principal Investigator |
Shirakawa Hideki 電気通信大学, 大学院情報理工学研究科, 准教授 (40241070)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | カルシウムシグナリング / オプトジェネティクス / イノシトール三リン酸 / 細胞内カルシウム |
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| Outline of Final Research Achievements |
Protein-based molecular tools were developed, aiming to establish the IP3 clamp method, a novel technique to control the intracellular IP3 concentrations in living cells by light. It was shown that the combination of human phospholipase C and plant photoreceptor proteins could give the photoactivatability to an IP3-producing enzyme. We also developed fluorescent IP3 probes that have favorable excitation/emission wavelengths to be used with photoactivatable enzymes in the IP3 clamp system.
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Report
(3 results)
Research Products
(7 results)
