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Development of SOI detector adopted for recording single molecular dynamics of proteins

Research Project

Project/Area Number 15K15030
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionUniversity of Fukui

Principal Investigator

Shimizu Hirofumi  福井大学, 学術研究院医学系部門, 講師 (50324158)

Co-Investigator(Renkei-kenkyūsha) Yasuo Arai  高エネルギー加速器研究機構, 教授 (90167990)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords1分子計測 / X線回折 / 蛋白質 / X線
Outline of Final Research Achievements

Single-molecular conformational changes of proteins have been recorded by diffracted X-ray tracking method in which the diffraction spots from a gold nanocrystal, attached to the protein, were tacked on the two dimensional X-ray detector. In some cases the entire motions were not recorded because the size of X-ray receiving surface was insufficient. Aiming to resolve this issue we examined whether the Silicon-On-Insulator (SOI) sensor whose pixel size is very small (~17micron) can be used for or not.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Temperature-Jump effects on the single molecular dynamics of the KcsA Potassium2017

    • Author(s)
      Hirofumi Shimizu, Masayuki Iwamoto
    • Journal Title

      J. Physiol. Sci.

      Volume: 67 Suppl

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Specific interactions between alkali metal cations and the KcsA channel studied using ATR-FTIR spectroscopy2015

    • Author(s)
      Y. Furutani, H. Shimizu, Y. Asai, S. Oiki, H. Kndori
    • Journal Title

      Biophysics and Physicobiology

      Volume: 12 Issue: 0 Pages: 37-45

    • DOI

      10.2142/biophysico.12.0_37

    • NAID

      130005098800

    • ISSN
      2189-4779
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Surface-enhanced IR absorption spectroscopy of the KcsA potassium channel upon application of an2015

    • Author(s)
      A. Yamakata, H. Shimizu, S. Oiki
    • Journal Title

      Phys Chem Chem Phys.

      Volume: 17 Issue: 33 Pages: 21104-21111

    • DOI

      10.1039/c5cp02681d

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] KcsAカリウムイオンチャネルの1分子運動に対する試料温度ジャンプの影響2017

    • Author(s)
      清水啓史、岩本真幸
    • Organizer
      第94回日本生理学会
    • Place of Presentation
      アクトシティ浜松、浜松
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Single-Molecule Fluctuations and Conformational Changes of KcsA Potassium2017

    • Author(s)
      Hirofumi Shimizu
    • Organizer
      The 8th Japan-Taiwan symposium on Nanomedicine
    • Place of Presentation
      Academia Sinica, Taiwan
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] X線1 分子動態計測法への試料温度ジャンプシステムの導入2016

    • Author(s)
      清水啓史、岩本真幸
    • Organizer
      第54回日本生物物理学会
    • Place of Presentation
      つくば国際会議場、つくば
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Photoresist Micro-Chamber for the Diffracted X-ray Tracking Method Recording Single-Molecule Conformational Changes2016

    • Author(s)
      Kio Tahara, Yoshikazu Hirai, Hirofumi Shimizu, Toshiyuki
    • Organizer
      The 30th EUROSENSORS (EUROSENSORS XXX)
    • Place of Presentation
      Butapest, Hungary
    • Year and Date
      2016-09-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イオンチャネルの1分子構造変化計測2016

    • Author(s)
      清水啓史
    • Organizer
      第一回イオンチャネル研究会
    • Place of Presentation
      福岡大学、福岡
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 蛋白質の分子揺らぎと構造変化を計測するX線1分子動態計測法の開発2015

    • Author(s)
      清水啓史
    • Organizer
      第53会日本生物物理学会年会、シンポジウム「動的生命科学を拓く新発想測定技術」(招待講演)
    • Place of Presentation
      金沢
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 3次元微細加工技術を応用したX線1分子動態計測用低ノイズマイクロチャンバの開発2015

    • Author(s)
      田原樹生,平井義和,清水啓史,土屋智由,田畑 修
    • Organizer
      電気学会総合研究会 E部門総合研究会
    • Place of Presentation
      福岡県 九州大学医学部百年講堂
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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