Investigation of a novel lipase-like transmembrane protein for the discovery of drugs to regulate the epidermal barrier function

• Project/Area Number: 15K15053
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Osaka University
• Principal Investigator: Kanai Yoshikatsu 大阪大学, 医学系研究科, 教授 (60204533)
• Co-Investigator(Kenkyū-buntansha): 永森 收志 大阪大学, 医学系研究科, 准教授 (90467572) ; 大垣 隆一 大阪大学, 医学系研究科, 助教 (20467525) ; 奥田 傑 大阪大学, 医学系研究科, 助教 (50511846) ; 中込 咲綾 大阪大学, 微生物病研究所, 特任研究員(常勤) (60423894)
• Research Collaborator: ISHIKAWA Junko
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 表皮バリア / 角質細胞間脂質ラメラ / リパーゼ / リピドミクス

Outline of Final Research Achievements

The intercellular spaces in stratum corneum are filled with the lamellae of lipids, the structural entity of the epidermal barrier. We previously found that a novel lipase-like protein LIPX is essential for the epidermal barrier function. In this study, by using a LIPX gene knockout mouse strain, we investigated the mechanisms by which LIPX contributes to the formation of the lipid lamellae in the stratum corneum. We revealed that, in the absence of LIPX, the intercellular lipid lamellae formed in the stratum corneum were structurally abnormal. This defect was accompanied with a drastic decrease of ceramides that are essential for the epidermal barrier. We also demonstrated that LIPX is a transmembrane protein specifically expressed in the epidermis. The results of this study contribute to understanding the essential roles of LIPX in the epidermal barrier function and to developing novel medicines to cure the diseases with dysfunction of the epidermal barrier function.

Research Products

• [Journal Article] (18)F-FBPA as a tumor-specific probe of L-type amino acid transporter 1 (LAT1): a comparison study with (18)F-FDG and (11)C-Methionine PET. (2017)
  • Author(s): Watabe T, Ikeda H, Nagamori S, Wiriyasermkul P, Tanaka Y, Naka S, Kanai Y, Hagiwara K, Aoki M, Shimosegawa E, Kanai Y, Hatazawa J.
  • Journal Title: Eur J Nucl Med Mol Imaging. Volume: 44 Issue: 2 Pages: 321-331
  • DOI: 10.1007/s00259-016-3487-1
  • Peer Reviewed
• [Journal Article] Increased Dynamics of Tricarboxylic Acid Cycle and Glutamate Synthesis in Obese Adipose Tissue: IN VIVO METABOLIC TURNOVER ANALYSIS. (2017)
  • Author(s): Nagao H, Nishizawa H, Bamba T, Nakayama Y, Isozumi N, Nagamori S, Kanai Y, Tanaka Y, Kita S, Fukuda S, Funahashi T, Maeda N, Fukusaki E, Shimomura I.
  • Journal Title: J Biol Chem. Volume: 292 Issue: 11 Pages: 4469-4483
  • DOI: 10.1074/jbc.m116.770172
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic Significance of the Expression of CD98 (4F2hc) in Gastric Cancer. (2017)
  • Author(s): Satoh T, Kaira K, Takahashi K, Takahashi N, Kanai Y, Asao T, Horiguchi J, Oyama T.
  • Journal Title: Anticancer Res. Volume: 37 Pages: 631-636
  • Peer Reviewed
• [Journal Article] Structure-activity relationship of a novel series of inhibitors for cancer type transporter L-type amino acid transporter 1 (LAT1). (2017)
  • Author(s): Kongpracha P, Nagamori S, Wiriyasermkul P, Tanaka Y, Kaneda K, Okuda S, Ohgaki R, Kanai Y.
  • Journal Title: J Pharmacol Sci. Volume: 133 Issue: 2 Pages: 96-102
  • DOI: 10.1016/j.jphs.2017.01.006
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Essential roles of L-type amino acid transporter 1 in syncytiotrophoblast development by presenting fusogenic 4F2hc. (2017)
  • Author(s): Ohgaki R, Ohmori T, Hara S, Nakagomi S, Kanai-Azuma M, Kaneda-Nakashima K, Okuda S, Nagamori S, Kanai Y.
  • Journal Title: Mol Cell Biol. Volume: 印刷中 Issue: 11
  • DOI: 10.1128/mcb.00427-16
  • Peer Reviewed / Open Access
• [Journal Article] Ultrastructural immunohistochemical study of L-type amino acid transporter 1-4F2 heavy chain in tumor microvasculatures of N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder carcinoma. (2017)
  • Author(s): Kume E, Mutou T, Kansaku N, Takahashi H, Wempe MF, Ikegami M, Kanai Y, Endou H, Wakui S.
  • Journal Title: Microscopy (Oxf). Volume: 24 Issue: 3 Pages: 1-6
  • DOI: 10.1093/jmicro/dfx008
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer. (2017)
  • Author(s): Ohshima K, Nojima S, Tahara S, Kurashige M, Hori Y, Hagiwara K, Okuzaki D, Oki S, Wada N, Ikeda JI, Kanai Y, Morii E.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 45504-45504
  • DOI: 10.1038/srep45504
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Utilization of Liver Microsomes to Estimate Hepatic Intrinsic Clearance of Monoamine Oxidase Substrate Drugs in Humans. (2017)
  • Author(s): Masuo Y, Nagamori S, Hasegawa A, Hayashi K, Isozumi N, Nakamichi N, Kanai Y, Kato Y.
  • Journal Title: Pharm Res Volume: 印刷中 Issue: 6 Pages: 1233-1243
  • DOI: 10.1007/s11095-017-2140-4
  • NAID: 120006306246
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regulation of amino acid transporter trafficking by mTORC1 in primary human trophoblast cells is mediated by the ubiquitin ligase Nedd4-2. (2016)
  • Author(s): Rosario FJ, Dimasuay KG, Kanai Y, Powell TL, Jansson T.
  • Journal Title: Clin Sci (Lond). Volume: 130 Issue: 7 Pages: 499-512
  • DOI: 10.1042/cs20150554
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Structure-activity relations of leucine derivatives reveal critical moieties for cellular uptake and activation of mTORC1-mediated signaling. (2016)
  • Author(s): Nagamori S, Wiriyasermkul P, Okuda S, Kojima N, Hari Y, Kiyonaka S, Mori Y, Tominaga H, Ohgaki R, Kanai Y.
  • Journal Title: Amino Acids. Volume: 48 Issue: 4 Pages: 279-286
  • DOI: 10.1007/s00726-015-2158-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Interaction of the Sodium/Glucose Cotransporter (SGLT) 2 inhibitor Canagliflozin with SGLT1 and SGLT2: Inhibition kinetics, sidedness of action, and transporter-associated incorporation accounting for its pharmacodynamic and pharmacokinetic features. (2016)
  • Author(s): Ohgaki R, Wei L, Yamada K, Hara T, Kuriyama C, Okuda S, Ueta K, Shiotani M, Nagamori S, Kanai Y.
  • Journal Title: J Pharmacol Exp Ther. Volume: 358 Issue: 1 Pages: 94-102
  • DOI: 10.1124/jpet.116.232025
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy. (2016)
  • Author(s): Takamatsu S, Shimomura M, Kamada Y, Maeda H, Sobajima T, Hikita H, Iijima M, Okamoto Y, Misaki R, Fujiyama K, Nagamori S, Kanai Y, Takehara T, Ueda K, Kuroda S, Miyoshi E.
  • Journal Title: Glycobiology. Volume: 26 Pages: 1180-1189
  • DOI: 10.1093/glycob/cww067
  • Peer Reviewed
• [Journal Article] Efficacy of system l amino acid transporter 1 inhibition as a therapeutic target in esophageal squamous cell carcinoma. (2016)
  • Author(s): Ohshima Y, Kaira K, Yamaguchi A, Oriuchi N, Tominaga H, Nagamori S, Kanai Y, Yokobori T, Miyazaki T, Asao T, Tsushima Y, Kuwano H, Ishioka NS.
  • Journal Title: Cancer Sci. Volume: 107 Issue: 10 Pages: 1499-1505
  • DOI: 10.1111/cas.13021
  • Peer Reviewed / Open Access
• [Journal Article] Expression of a human NPT1/SLC17A1 missense variant which increases urate export. (2016)
  • Author(s): Sakiyama M, Matsuo H, Nagamori S, Ling W, Kawamura Y, Nakayama A, Higashino T, Chiba T, Ichida K, Kanai Y, Shinomiya N.
  • Journal Title: Nucleosides Nucleotides Nucleic Acids. Volume: 35 Pages: 536-542
  • Peer Reviewed
• [Journal Article] Impaired Amino Acid Transport at the Blood Brain Barrier Is a Cause of Autism Spectrum Disorder. (2016)
  • Author(s): Tarlungeanu DC, Deliu E, Dotter CP, Kara M, Janiesch PC, Scalise M, Galluccio M, Tesulov M, Morelli E, Sonmez FM, Bilguvar K, Ohgaki R, Kanai Y, Johansen A, Esharif S, Ben-Omran T, Topcu M, Schlessinger A, Indiveri C, Duncan KE, Caglayan AO, Gunel M, Gleeson JG, Novarino G.
  • Journal Title: Cell. Volume: 167 Issue: 6 Pages: 1481-1494
  • DOI: 10.1016/j.cell.2016.11.013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Novel cystine transporter in renal proximal tubule identified as a missing partner of cystinuria-related plasma membrane protein rBAT/SLC3A1. (2016)
  • Author(s): Nagamori S, Wiriyasermkul P, Guarch ME, Okuyama H, Nakagomi S, Tadagaki K, Nishinaka Y, Bodoy S, Takafuji K, Okuda S, Kurokawa J, Ohgaki R, Nunes V, Palacín M, Kanai Y.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 113 Issue: 3 Pages: 775-780
  • DOI: 10.1073/pnas.1519959113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Specific transport of 3-fluoro-l-α-methyl-tyrosine by LAT1 explains its specificity to malignant tumors in imaging. (2016)
  • Author(s): Wei L, Tominaga H, Ohgaki R, Wiriyasermkul P, Hagiwara K, Okuda S, Kaira K, Oriuchi N, Nagamori S, Kanai Y.
  • Journal Title: Cancer Sci. Volume: 107 Issue: 3 Pages: 347-352
  • DOI: 10.1111/cas.12878
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transport of 3-fluoro-l-α-methyl-tyrosine (FAMT) by organic ion transporters explains renal background in [(18)F]FAMT positron emission tomography. (2016)
  • Author(s): Wei L, Tominaga H, Ohgaki R, Wiriyasermkul P, Hagiwara K, Okuda S, Kaira K, Kato Y, Oriuchi N, Nagamori S, Kanai Y.
  • Journal Title: J Pharmacol Sci. Volume: 130 Issue: 2 Pages: 101-109
  • DOI: 10.1016/j.jphs.2016.01.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clinicopathological significance of LAT1 and ASCT2 in patients with surgically resected esophageal squamous cell carcinoma. (2016)
  • Author(s): Honjo H, Kaira K, Miyazaki T, Yokobori T, Kanai Y, Nagamori S, Oyama T, Asao T, Kuwano H.
  • Journal Title: J Surg Oncol. Volume: 113 Issue: 4 Pages: 381-9
  • DOI: 10.1002/jso.24160
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SGLT2阻害薬の特徴：基礎研究の観点から (2016)
  • Author(s): 金井好克
  • Journal Title: Progress in Medicine Volume: 36 Pages: 183-188
• [Journal Article] Up-Regulation of LAT1 during Antiandrogen Therapy Contributes to Progression in Prostate Cancer Cells (2015)
  • Author(s): Xu M, Sakamoto S, Matsushima J, Kimura T, Ueda T, Mizokami A, Kanai Y, Ichikawa T.
  • Journal Title: J Urol Volume: 195 Issue: 5 Pages: 1588-1597
  • DOI: 10.1016/j.juro.2015.11.071
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of a Widely Usable Amino Acid Tracer: 76Br-α-Methyl-Phenylalanine for Tumor PET Imaging. (2015)
  • Author(s): Hanaoka H, Ohshima Y, Suzuki Y, Yamaguchi A, Watanabe S, Uehara T, Nagamori S, Kanai Y, Ishioka NS, Tsushima Y, Endo K, Arano Y
  • Journal Title: J Nucl Med. Volume: 56 Issue: 5 Pages: 791-797
  • DOI: 10.2967/jnumed.114.152215
  • Peer Reviewed
• [Journal Article] Expression of Amino Acid Transporters (LAT1 and ASCT2) in Patients with Stage III/IV Laryngeal Squamous Cell Carcinoma. (2015)
  • Author(s): Nikkuni O, Kaira K, Toyoda M, Shino M, Sakakura K, Takahashi K, Tominaga H, Oriuchi N, Suzuki M, Iijima M, Asao T, Nishiyama M, Nagamori S, Kanai Y, Oyama T, Chikamatsu K.
  • Journal Title: Pathol Oncol Res Volume: 21 Issue: 4 Pages: 1175-1181
  • DOI: 10.1007/s12253-015-9954-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea. (2015)
  • Author(s): Uetsuka S, Ogata G, Nagamori S, Isozumi N, Nin F, Yoshida T, Komune S, Kitahara T, Kikkawa Y, Inohara H, Kanai Y, Hibino H.
  • Journal Title: Eur J Neurosci. Volume: 42 Issue: 3 Pages: 1984-2002
  • DOI: 10.1111/ejn.12973
  • Peer Reviewed
• [Journal Article] L-type amino acid transporter-1 and CD98 expression in bone and soft tissue tumors. (2015)
  • Author(s): Koshi H, Sano T, Handa T, Yanagawa T, Saitou K, Nagamori S, Kanai Y, Takagishi K, Oyama T.
  • Journal Title: Pathol Int. Volume: 65 Issue: 9 Pages: 460-470
  • DOI: 10.1111/pin.12323
  • Peer Reviewed
• [Journal Article] Increased placental nutrient transport in a novel mouse model of maternal obesity with fetal overgrowth. (2015)
  • Author(s): Rosario FJ, Kanai Y, Powell TL, Jansson T.
  • Journal Title: Obesity (Silver Spring). Volume: 23 Issue: 8 Pages: 1663-1670
  • DOI: 10.1002/oby.21165
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prognostic significance of L-type amino acid transporter 1 (LAT1) expression in cutaneous melanoma. (2015)
  • Author(s): Shimizu A, Kaira K, Kato M, Yasuda M, Takahashi A, Tominaga H, Oriuchi N, Nagamori S, Kanai Y, Oyama T, Asao T, Ishikawa O.
  • Journal Title: Melanoma Res. Volume: 25 Issue: 5 Pages: 399-405
  • DOI: 10.1097/cmr.0000000000000181
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional identification of SLC43A3 as an equilibrative nucleobase transporter involved in purine salvage in mammals. (2015)
  • Author(s): Furukawa J, Inoue K, Maeda J, Yasujima T, Ohta K, Kanai Y, Takada T, Matsuo H, Yuasa H
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 15057-15057
  • DOI: 10.1038/srep15057
  • Peer Reviewed / Open Access
• [Journal Article] 疾患起因性変異ノックインマウスを用いた日本人型シスチン尿症変異の特性解析 (2015)
  • Author(s): 永森 收志, 河本 泰治, Wiriyasermkul Pattama, 中込 咲綾, 金井 好克
  • Journal Title: 日本尿路結石症学会誌 Volume: 14 Pages: 151-153
• [Journal Article] CD98 as a novel prognostic indicator for patients with stage III/IV hypopharyngeal squamous cell carcinoma. (2014)
  • Author(s): Toyoda M, Kaira K, Shino M, Sakakura K, Takahashi K, Takayasu Y, Tominaga H, Oriuchi N, Nikkuni O, Suzuki M, Iijima M, Tsukamoto N, Nagamori S, Kanai Y, Oyama T, Chikamatsu K.
  • Journal Title: Head Neck. Volume: in press Issue: 11 Pages: 1-6
  • DOI: 10.1002/hed.23797
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 栄養食品成分の吸収・排出を担う膜輸送体の網羅的定量プロテオミクスによる変動解析Comprehensive proteomics reveals alteration of membrane transport proteins responsible for absorption and excretion of nutrients and food ingredients. (2017)
  • Author(s): 永森收志
  • Organizer: 2017年度 日本農芸化学会年会 シンポジウム「栄養食品成分の吸収と排出～膜を超える仕組み」
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
  • Invited
• [Presentation] SGLT2阻害薬カナグリフロジンのヒトSGLT1およびSGLT2に対する作用キネティクスと阻害メカニズムの検討 (2017)
  • Author(s): 大垣隆一、Ling Wei、山田和徳、原大樹、栗山千亜紀、奥田傑、植田喜一郎、Laurent Leclercq、Rao N.V.S. Mamidi、塩谷正治、永森收志、金井好克
  • Organizer: 第90回 日本薬理学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2017-03-14
• [Presentation] アミノ酸トランスポーターLAT1によるロイシン誘導体の認識とmTORC1シグナル活性化の関係 (2017)
  • Author(s): 奥田傑、Pattama Wiriyasermkul、大垣隆一、永森收志、金井好克
  • Organizer: 第90回 日本薬理学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2017-03-14
• [Presentation] 有機アニオントランスポーターOAT1に対する芳香族アミノ酸誘導体の構造活性相関：イメージングプローブの腎臓におけるバックグラウンド集積を誘起する分子内構造の同定 (2017)
  • Author(s): 魏玲、大垣隆一、富永英之、兼田加珠子、奥田傑、永森收志、金井好克
  • Organizer: 第90回 日本薬理学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2017-03-14
• [Presentation] 腫瘍細胞型アミノ酸トランスポーターリガンドの構造活性相関から見えてくる創薬標的としての意義 (2017)
  • Author(s): 金井 好克
  • Organizer: 第90回日本薬理学会年会シンポジウム「トランスポーターの分子同定から展開する創薬研究」
  • Place of Presentation: 長崎
  • Year and Date: 2017-03-14
  • Invited
• [Presentation] Amino acid transporter LAT1 as a novel molecular target for cancer diagnosis and therapeutics (2017)
  • Author(s): Yoshikatsu Kanai
  • Organizer: AMED-ALBERTA WORKSHOP FOR MEDICAL INNOVATION
  • Place of Presentation: Calgary, Alberta, Canada
  • Year and Date: 2017-02-24
  • Int'l Joint Research / Invited
• [Presentation] 膜輸送複合体の機能的再構成人工膜－生体界面研究のモデル系を目指して (2017)
  • Author(s): 永森收志
  • Organizer: 第6回 生体界面研究会
  • Place of Presentation: 金沢
• [Presentation] がん細胞型アミノ酸トランスポーターを標的とした新規作用機序の抗悪性腫瘍薬と汎用性の高いがん特異的PETプロープの開発 (2017)
  • Author(s): 金井 好克
  • Organizer: 平成28年度創薬シーズ事業化コンペティション
  • Place of Presentation: 大阪
• [Presentation] Pharmacology to innovative drug development: cancer therapeutics and diagnosis targeting amino acid transporters (2017)
  • Author(s): Yoshikatsu Kanai
  • Organizer: Campus Asia International Symposium (Ⅱ)
  • Place of Presentation: 大阪
  • Int'l Joint Research / Invited
• [Presentation] アミノ酸トランスポーターLAT1は胎盤合胞体性栄養膜の形成に必須である：膜融合関連因子4F2hcの細胞膜提示による細胞融合への寄与 (2016)
  • Author(s): 大垣 隆一、大森 崇弘、原 早織、中込 咲綾、金井 正美、兼田 加珠子、奥田 傑、永森 收志、金井 好克
  • Organizer: 第39回 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] 腎近位尿細管に残されていた第二のシスチントランスポーターはシスチン尿症関連タンパク質rBATの未知なる結合相手だった (2016)
  • Author(s): 永森收志
  • Organizer: 第89回日本生化学会大会、シンポジウム「膜輸送体学の「温故知新」～長年の謎の解明をめざして」
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
  • Invited
• [Presentation] Transporter and urolithiasis (2016)
  • Author(s): Yoshikatsu Kanai
  • Organizer: 13th International Symposium on Urolithiasis
  • Place of Presentation: 千葉
  • Year and Date: 2016-07-19
  • Int'l Joint Research / Invited
• [Presentation] Inhibition kinetics, sidedness of action, and transporter-mediated uptake of the sodium/glucose cotransporter (SGLT) 2 inhibitor canagliflozin: implications for its pharmacodynamic and pharmacokinetic features (2016)
  • Author(s): Ling Wei, Ryuichi Ohgaki, Kazunori Yamada, Taiki Hara, Chiaki Kuriyama, Suguru Okuda, Kiichiro Ueta, Masaharu shiotani, Shushi Nagamori and Yoshikatsu Kanai
  • Organizer: International and Interdisciplinary Symposium 2016 “Towards a New Era of Cardiovascular Research”
  • Place of Presentation: 東京
  • Year and Date: 2016-07-12
  • Int'l Joint Research
• [Presentation] Significance of the comprehensive and quantitative proteomics of membrane transport proteins in drug discovery and in silico modeling. (2016)
  • Author(s): Shushi Nagamori
  • Organizer: International and Interdisciplinary Symposium 2016“Towards a New Era of Cardiovascular Research”
  • Place of Presentation: 東京
  • Year and Date: 2016-07-12
  • Int'l Joint Research / Invited
• [Presentation] Inhibition kinetics, sidedness of action, and transporter-mediated uptake of the sodium/glucose cotransporter (SGLT) 2 inhibitor canagliflozin: implications for its pharmacodynamic and pharmacokinetic features (2016)
  • Author(s): Ohgaki R, Wei L, Yamada K, Hara T, Kuriyama C, Okuda S, Ueta K, Leclercq L, Mamidi RN, Shiotani M, Nagamori S, Kanai Y
  • Organizer: 76th American Diabetes Association Scientific Sessionｓ
  • Place of Presentation: New Orleans, Louisiana, US
  • Year and Date: 2016-06-10
  • Int'l Joint Research
• [Presentation] Novel Cystine Transporter in Renal Proximal Tubule Identied as a “Missing Partner” of Cystinuria-Related Membrane Protein rBAT/SLC3A1. (2016)
  • Author(s): Nagamori S, Wiriyasermkul P, Okuyama K, Nakagomi S, Okuda S, Ohgaki R, Kanai Y.
  • Organizer: The Kaback Symposium
  • Place of Presentation: Bethesda, Maryland, US
  • Year and Date: 2016-06-09
  • Int'l Joint Research / Invited
• [Presentation] Novel Cystine Transporter in Renal Proximal Tubule Identied as a “Missing Partner” of Cystinuria-Related Membrane Protein rBAT/SLC3A1. (2016)
  • Author(s): Nagamori S, Wiriyasermkul P, Okuyama K, Nakagomi S, Okuda S, Ohgaki R, Kanai Y.
  • Organizer: Experimental Biology 2016
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2016-04-22
  • Int'l Joint Research
• [Presentation] Novel Cystine Transporter in Renal Proximal Tubule Identied as a “Missing Partner” of Cystinuria-Related Membrane Protein rBAT/SLC3A1. (2016)
  • Author(s): 3.Nagamori S, Wiriyasermkul P, Okuyama K, Nakagomi S, Okuda S, Ohgaki R, Kanai Y.
  • Organizer: Experimental Biology 2016
  • Place of Presentation: San Diego, CA, US
  • Year and Date: 2016-04-02
  • Int'l Joint Research
• [Presentation] SGLT2阻害薬が再認識させた腎尿細管トランスポーターの創薬標的としての意義 (2016)
  • Author(s): 金井好克
  • Organizer: 第８９回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-09
  • Invited
• [Presentation] Significance of comprehensive and quantitative proteomics of membrane transport proteins in drug discovery. 創薬研究における膜輸送体の網羅的解析と定量解析の意義 (2016)
  • Author(s): Nagamori S
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-09
  • Invited
• [Presentation] A renal tubular transporter mediating reabsorption of β-hydroxybutyrate: a potential target of drugs increasing urinary ketone body excretion. ケトン体排泄促進薬の標的としてのβ-ヒドロキシ酪酸再吸収を担う尿細管トランスポーター (2016)
  • Author(s): Nagamori S
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-09
  • Invited
• [Presentation] Interaction of potential hepatotoxic ingredients of Traditional Chinese Medicine with liver drug transporters OATP1B1 and MDR1 (2016)
  • Author(s): Lili Quan，Zijie Song, Yueqing Zhou, Ryuichi Ohgaki, Shushi Nagamori, Xin He, Yoshikatsu Kanai
  • Organizer: 第63回 日本生化学会 近畿支部例会学会
  • Place of Presentation: 神戸
• [Presentation] Interaction of halogenated aromatic amino acid probes for tumor imaging with renal organic anion transporter OAT1: the mechanisms of renal accumulation of the imaging probes (2016)
  • Author(s): Ling Wei、大垣 隆一、富永 英之、Quan Lili、兼田（中島）加珠子、奥田 傑、永森 收志、金井 好克
  • Organizer: 第129回 日本薬理学会近畿部会
  • Place of Presentation: 広島
• [Presentation] PEG結合リン脂質型蛍光分子プローブによる細胞膜表層pHのイメージング (2016)
  • Author(s): 大垣 隆一、寺村 裕治、林 大智、永森 收志、高井 まどか、金井 好克
  • Organizer: 第129回 日本薬理学会近畿部会
  • Place of Presentation: 広島
• [Presentation] 栄養素トランスポーターを標的とした病態代謝制御：SGLT2阻害薬の最近の知見とアミノ酸・有機酸トランスポーターの創薬標的としての可能性 (2016)
  • Author(s): 金井 好克
  • Organizer: 第164回 分泌セミナー
  • Place of Presentation: 東京
  • Invited
• [Presentation] PEG結合リン脂質型蛍光分子プローブの 細胞膜表層pHイメージングツールとしての有用性の検討 (2016)
  • Author(s): 大垣 隆一、寺村 裕治、林 大智、永森 收志、高井 まどか、金井 好克
  • Organizer: H28年度 生理研研究会（第5回 生体界面研究会）
  • Place of Presentation: 岡崎
• [Presentation] トランスポーターを標的とした創薬 (2016)
  • Author(s): 金井 好克
  • Organizer: 千里ライフサイエンスセミナーK2「トランスポーターと創薬：構造と病態からのアプローチ」
  • Place of Presentation: 大阪
  • Invited
• [Presentation] Novel cystine transporter in renal proximal tubule identified as a “missing partner” of cystinuria-related membrane protein rBAT/SLC3A1 (2016)
  • Author(s): Nagamori S, Wiriyasermkul P, Espino Guarch M, Okuyama K, Nakagomi S, Okuda S, Ohgaki R, Palacin M, Kanai Y.
  • Organizer: 第25回 日本尿路結石症学会
  • Place of Presentation: 千葉
• [Presentation] Amino acid transporters as targets for cancer-selective drug delivery and anti-cancer therapeutics (2016)
  • Author(s): Yoshikatsu Kanai
  • Organizer: 2nd Drug Transporters Forum
  • Place of Presentation: Lanzhou, China
  • Int'l Joint Research / Invited
• [Presentation] がん細胞アミノ酸トランスポーターを標的としたがんの検出と細胞活性制御 (2016)
  • Author(s): 金井 好克
  • Organizer: 日本農芸化学会平成28 年度関西支部大会シンポジウム「アミノ酸研究の最前線」
  • Place of Presentation: 大津
  • Invited
• [Presentation] アミノ酸トランスポーターを標的とした創薬研究 (2016)
  • Author(s): 永森收志
  • Organizer: 第一回名古屋大学医薬系3部局交流シンポジウム
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] アミノ酸トランスポーターLAT1による基質認識とmTORC1シグナル活性化に必要なロイシンの構造部位の決定 (2016)
  • Author(s): 奥田 傑, Pattama Wiriyasermkul, 大垣 隆一, 永森 收志, 金井 好克
  • Organizer: 第130回 日本薬理学会近畿部会
  • Place of Presentation: 京都
• [Presentation] アミノ酸トランスポーターLAT1阻害によるがん転移抑制効果の検討 (2016)
  • Author(s): 兼田 加珠子, 魏 玲, Pornparn Kongpracha, 大垣 隆一, 永森 收志, 金井 好克
  • Organizer: 第130回 日本薬理学会近畿部会
  • Place of Presentation: 京都
• [Presentation] がん細胞のアミノ酸トランスポーターを標的としたがんPET 診断と抗腫瘍治療の開発 (2015)
  • Author(s): 金井好克
  • Organizer: 第２回臨床アミノ酸研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-12-05
  • Invited
• [Presentation] Non-competitive inhibitors arrest the alternating-access mechanism of amino acid transporters. (2015)
  • Author(s): Nagamori S
  • Organizer: 第38回分子生物学会年会・第88回生化学会大会合同大会（BMB2015）
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Leucine transported by a cancer-type amino acid transporter LAT1 affects multiple cellular processes in pancreatic cancer cells. (2015)
  • Author(s): Pornparn Kongpracha, Pattama Wiriyasermkul, Noriyoshi Isozumi, Kazuko Kaneda-Nakashima, Suguru Okuda, Ryuichi Ohgaki, Shushi Nagamori, Yoshikatsu Kanai
  • Organizer: 第38回分子生物学会年会・第88回生化学会大会合同大会（BMB2015）
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] アミノ酸輸送体阻害薬は新規抗がん薬として有用である Inhibitor of amino acid transporter is useful as a novel anti-cancer drug (2015)
  • Author(s): 兼田（中島）加珠子、疋田隼人、Pornparn Kongpracha、Ling Wei、大垣隆一、 永森收志、竹原徹郎、金井好克
  • Organizer: 第38回分子生物学会年会・第88回生化学会大会合同大会（BMB2015）
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] HEK293T細胞のアミノ酸シグナルによるmTORC1活性化の特性についての検討 Properties of mTORC1 regulation by amino acid signaling in HEK293T cells (2015)
  • Author(s): 大垣 隆一、永森 收志、ウィリヤサムクン パッタマ、萩原 浩平、金井 好克
  • Organizer: 第38回分子生物学会年会・第88回生化学会大会合同大会（BMB2015）
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Transporters in pharmacology and molecular target drug discovery (2015)
  • Author(s): 金井好克
  • Organizer: APSA-ASCEPT Joint Scientific Meeting 2015
  • Place of Presentation: Hobart, Australia
  • Year and Date: 2015-11-29
  • Int'l Joint Research / Invited
• [Presentation] SGLT2阻害薬の作用とメカニズム (2015)
  • Author(s): 金井好克
  • Organizer: 日本糖尿病合併症学会イブニングセミナー
  • Place of Presentation: 名古屋
  • Year and Date: 2015-11-27
  • Invited
• [Presentation] Na+/グルコース共輸送体2（SGLT2）阻害薬の作用キネティクスと阻害メカニズム (2015)
  • Author(s): 大垣 隆一、Wei Ling、永森 收志、山田 和徳、原 大樹、栗山 千亜紀、植田 喜一郎、塩谷 正治、金井 好克
  • Organizer: 第128回日本薬理学会近畿部会
  • Place of Presentation: 大阪
  • Year and Date: 2015-11-20
• [Presentation] アミノ酸トランスポーター阻害薬による抗腫瘍薬作用増強効果についての検討 (2015)
  • Author(s): 兼田加珠子、Pornparn Kongpracha、Ling Wei、大垣 隆一、 永森 收志、金 井 好克
  • Organizer: 第37回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-11-19
• [Presentation] プロテオミクスによるアミノ酸輸送と介在シグナルの全体像の解明 (2015)
  • Author(s): 永森收志
  • Organizer: 日本アミノ酸学会第9回学術大会（JSAAS2015）
  • Place of Presentation: 滋賀
  • Year and Date: 2015-10-23
  • Invited
• [Presentation] Investigation of regulation mechanisms of cancer specific amino acid transporter, LAT1 がん特異的アミノ酸トランスポーターLAT1の制御システムの探索 (2015)
  • Author(s): 兼田（中島）加珠子、永森 收志、 金井 好克
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] 網羅的定量プロテオミクスと機能解析によるがん細胞株アミノ酸トランスポーターのプロファイリング (2015)
  • Author(s): 永森收志
  • Organizer: 平成27年度生理研研究会
  • Place of Presentation: 岡崎
  • Year and Date: 2015-09-15
  • Invited
• [Presentation] 網羅的プロテオーム解析が捉えるアミノ酸トランスポーターによる生体システムの制御 (2015)
  • Author(s): 永森收志
  • Organizer: 平成27年（第96回）日本栄養・食糧学会関東支部大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-05
  • Invited
• [Presentation] 疾患起因性変異ノックインマウスを用いた日本人型シスチン尿症変異の特性解析 (2015)
  • Author(s): 永森收志、 河本 泰治、Pattama Wiriyasermkul1、中込 咲綾、金井 好克
  • Organizer: 第25回日本尿路結石症学会
  • Place of Presentation: 旭川
  • Year and Date: 2015-08-28
• [Presentation] Amino acid transporters as targets for cancer-selective drug delivery and anti-cancer therapeutics (2015)
  • Author(s): 金井好克
  • Organizer: BioMedical Transporters Conference 2015
  • Place of Presentation: Lugano, Switzerland
  • Year and Date: 2015-08-09
  • Int'l Joint Research / Invited
• [Presentation] プロテオミクスと輸送機能測定によるがん細胞アミノ酸トランスポーターの網羅的解析 (2015)
  • Author(s): 永森收志、五十棲規義、Wiriyasermkul P、Kongpracha P、金井好克
  • Organizer: 日本プロテオーム学会 2015 年会
  • Place of Presentation: 熊本
  • Year and Date: 2015-07-23
• [Presentation] 生体界面構成因子の定量的解析に向けた膜タンパク質定量プロテオミクス (2015)
  • Author(s): 永森收志
  • Organizer: 平成27年度生理研研究会
  • Place of Presentation: 岡崎
  • Year and Date: 2015-07-16
  • Invited
• [Presentation] アミノ酸輸送体阻害薬による抗がん剤作用増強効果についての検討 (2015)
  • Author(s): 兼田 加珠子、Pornparn Kongpracha、Ling Wei、大垣 隆一、永森 收志、金 井 好克
  • Organizer: 第24回日本Cell Death学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-07-11
• [Presentation] がん特異的アミノ酸トランスポーターを標的としたがん診断法と治療法の開発 (2015)
  • Author(s): 永森收志
  • Organizer: 第31回日本DDS学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-02
  • Invited
• [Presentation] Transport of 3-fluoro-L-α-methyl-tyrosine (FAMT) by organic ion transporters: a possible cause of renal background in 18F-FAMT PET (2015)
  • Author(s): Wei Ling、富永 英之、Wiriyasermkul Pattama、大垣 隆一、萩原浩平、奥田 傑、永森 收志、金井 好克
  • Organizer: 第127回日本薬理学会近畿部会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-06-26
• [Presentation] アミノ酸トランスポーターとD-アミノ酸輸送 (2015)
  • Author(s): 永森收志
  • Organizer: 第15回日本蛋白質科学会
  • Place of Presentation: 徳島
  • Year and Date: 2015-06-24
  • Invited
• [Presentation] アミノ酸トランスポーターを標的とした創薬研究 (2015)
  • Author(s): 永森收志、金井好克
  • Organizer: 第10回日本トランスポーター研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-21
  • Invited
• [Presentation] がん細胞のアミノ酸トランスポーターを標的としたがん診断と抗腫瘍薬の開発 (2015)
  • Author(s): 金井好克
  • Organizer: 第４回谷根千腎カンファレンス
  • Place of Presentation: 東京
  • Year and Date: 2015-06-16
  • Invited
• [Presentation] Metabolomics and proteomics of knockout mice reveal physiological function of an orphan organic anion transporter. (2015)
  • Author(s): Nagamori S
  • Organizer: The 3rd Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications”
  • Place of Presentation: 淡路島
  • Year and Date: 2015-06-14
  • Int'l Joint Research / Invited
• [Presentation] SGLT2阻害薬カナグリフロジンのヒトSGLT2による輸送と細胞内外からの阻害作用の検討 (2015)
  • Author(s): 大垣隆一、Ling Wei、永森收志、山田和徳、原大樹、栗山千亜紀、植田喜一 郎、塩谷正治、金井好克
  • Organizer: 第58回日本糖尿病学会年次学術集会
  • Place of Presentation: 下関
  • Year and Date: 2015-05-21
• [Presentation] Inhibition of the LAT1-mediated leucine transport affects multiple cellular processes in pancreatic cancer cells. (2015)
  • Author(s): Pornparn Kongprach, Pattama Wiriyasermkul, Noriyoshi Isozumi, Printip Wongthai, Suguru Okuda, Kenjiro Tadagaki, Ryuichi Ohgaki, Shushi Nagamori, Yoshikatsu Kanai
  • Organizer: 第62回日本生化学会近畿支部例会
  • Place of Presentation: 滋賀
  • Year and Date: 2015-05-16