Development of analysis method of receptors using peptide wide affinity and resonance energy transfer
Project/Area Number |
15K15055
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
General pharmacology
|
Research Institution | Jichi Medical University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 広域親和性 / 受容体 / 光振動エネルギー移動 / ヘテロ受容体 / リガンド探索 / 発光タンパク質 / 蛍光タンパク質 / 共鳴エネルギー移動 / ルシフェラーゼ / ペプチドリガンド / 質量分析 / 薬理学的リネージ解析 |
Outline of Final Research Achievements |
Relationship between receptors and ligands has been considered as ‘key and lock’. Such strict structural requirement is not seen in our finding of synthetic peptide. We attempted to use our peptide with high affinities to a wide range of G-protein coupled receptors for analysis of orphan receptors. We selected candidate orphan receptors by pharmacological lineage analysis, which was developed by us previously. Although synthesis of florescent peptide holding similar affinity was difficult, variety of assay systems were successfully developed. Moreover, new findings were obtained on a receptor localization in a cell, regulatory mechanism of receptor internalization, and previously uncharacterized hereromer receptors. Our findings in this study can help further analyze and understand orphan receptors and heteromer receptors.
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Report
(4 results)
Research Products
(19 results)