| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Relationship between receptors and ligands has been considered as ‘key and lock’. Such strict structural requirement is not seen in our finding of synthetic peptide. We attempted to use our peptide with high affinities to a wide range of G-protein coupled receptors for analysis of orphan receptors. We selected candidate orphan receptors by pharmacological lineage analysis, which was developed by us previously. Although synthesis of florescent peptide holding similar affinity was difficult, variety of assay systems were successfully developed. Moreover, new findings were obtained on a receptor localization in a cell, regulatory mechanism of receptor internalization, and previously uncharacterized hereromer receptors. Our findings in this study can help further analyze and understand orphan receptors and heteromer receptors.
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