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Devolopment of a novel method to identify E3 ligase substrates

Research Project

Project/Area Number 15K15058
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionHokkaido University

Principal Investigator

WATANABE MASASHI  北海道大学, 医学研究科, 助教 (10632424)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsユビキチン / ユビキチンリガーゼ
Outline of Final Research Achievements

Since ubiquitin ligase (E3) has the substrate specificity of ubiquitination, identifications of specific substrates of each E3 enzymes and of their ubiquitination sites are important for understanding various biological phenomena. In recent years, Toczyski and colleagues have proposed a ligase trap method using a fusion probe of an ubiquitin-binding domain (UBA) and an E3, and Yoshida and colleagues have proposed a TR-TUBE method combining Tandem Ubiquitin Binding Entity (TUBE) and K-εGG-specific antibody. We attempted to develop a more advanced substrate identification method by adding some improvements in their methods and succeeded in improving the sensitivity. By using this method, it is expected that the functional analysis of E3 would be accelerated.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 4 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] TRIM proteins and diseases.2017

    • Author(s)
      Watanabe, M. and Hatakeyama, S
    • Journal Title

      J. Biochem.

      Volume: 161 Pages: 135-144

    • DOI

      10.1093/jb/mvw087

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The novel heart-specific RING finger protein 207 is involved in energy metabolism in cardiomyocytes.2016

    • Author(s)
      Mizushima, W., Takahashi , H., Watanabe, M., Kinugawa, S., Matsushima, S., Takada, S., Yokota, T., Furihata, T., Matsumoto, J., Tsuda, M., Chiba, I., Nagashima, S., Yanagi, S., Matsumoto, M., Nakayama, K.I., Tsutsui, H. and Hatakeyama, S.
    • Journal Title

      J. Mol. Cell. Cardiol.

      Volume: 100 Pages: 43-53

    • DOI

      10.1016/j.yjmcc.2016.09.013

    • NAID

      120006360069

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] p53 represses the transcription of snRNA genes by preventing the formation of little elongation complex2016

    • Author(s)
      Anwar, D., Takahashia, H., Watanabe, M., Suzuki, M., Fukuda, S., Hatakeyama, S.
    • Journal Title

      Biochim. Biophys. Acta-Gene Regul. Mech.

      Volume: 1859 Issue: 8 Pages: 975-982

    • DOI

      10.1016/j.bbagrm.2016.06.001

    • NAID

      120006328999

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] TRIM39 negatively regulates the NFκB-mediated signaling pathway through stabilization of Cactin2016

    • Author(s)
      Suzuki M, Watanabe M, Nakamaru Y, Takagi D, Takahashi H, Fukuda S, Hatakeyama S.
    • Journal Title

      Cell Mol Life Sci.

      Volume: 73 Issue: 5 Pages: 1085-1101

    • DOI

      10.1007/s00018-015-2040-x

    • NAID

      120005981342

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipocyte activator PPAR gamma.2015

    • Author(s)
      Watanabe, M., Takahashi, H., Saeki, Y., Ozaki, T., Itoh, S., Suzuki, M., Mizushima, W., Tanaka, K. and Hatakeyama, S.
    • Journal Title

      eLIFE

      Volume: 4

    • DOI

      10.7554/elife.05615

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ2016

    • Author(s)
      渡部 昌, 高橋秀尚, 畠山鎮次
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ユビキチンリガーゼTRIM23は非定型ポリユビキチン化によるPPARγの安定化を介して脂肪細胞分化を制御する2016

    • Author(s)
      渡部 昌, 高橋秀尚, 畠山鎮次
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      東北大学川内北キャンパス (宮城県・仙台市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] ユビキチンE3リガーゼTRIM23は非定型ポリユビキチン化によるPPARγの安定化を介して脂肪細胞分化を制御する2016

    • Author(s)
      渡部 昌, 高橋秀尚, 畠山鎮次
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都テルサ(京都府・京都市)
    • Year and Date
      2016-06-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] ユビキチンリガーゼTRIM23はPPARγの安定化を介して脂肪細胞分化を制御する2016

    • Author(s)
      渡部 昌, 高橋秀尚, 畠山鎮次
    • Organizer
      第89回日本内分泌学会学術総会
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2016-04-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] ユビキチンリガーゼTRIM23はPPARγの安定化を介して脂肪細胞分化を制御する2016

    • Author(s)
      渡部 昌, 高橋秀尚, 畠山鎮次
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Presentation] The ubiquitin E3 ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ.2015

    • Author(s)
      Masashi Watanabe
    • Organizer
      Cold Spring Harbor Laboratory Meeting on the Ubiquitin Family
    • Place of Presentation
      New York(USA)
    • Year and Date
      2015-04-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] Advances in Medicine and Biology. Volume 1202017

    • Author(s)
      Masashi Watanabe and Shigetsugu Hatakeyama
    • Total Pages
      23
    • Publisher
      Nova Science Publishers
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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