Comprehensive metalomics of cancer cells and tissues

• Project/Area Number: 15K15063
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Aichi Gakuin University (2016-2017); Nagoya University (2015)
• Principal Investigator: Takei Yoshifumi 愛知学院大学, 薬学部, 教授 (70362233)
• Co-Investigator(Renkei-kenkyūsha): HIRATA Takefumi 東京大学, 大学院理学系研究科, 教授 (10251612)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 癌転移 / 微量金属 / 質量分析 / 癌組織 / 脱タンパク質法 / 金属元素 / メタロミクス / スキルス胃癌 / 微量金属測定 / ICP-MS / 金属輸送タンパク質 / 遺伝子発現制御 / 癌治療 / 金属輸送蛋白質

Outline of Final Research Achievements

We successfully established to comprehensively measure the amounts of trace metals in cancer cells/tissues via ICP-MS method. Prior to ICP-MS, we performed deproteinization by trichloroacetic acid treatment. Using our method, we showed rubidium (atomic number 37) contents were significantly high in metastatic cell line from human scirrhous gastric cancers compared with the non-metastatic parental cell line. As the next experimental project, we obtained a precious cue to research the relationship between cancer metastasis and rubidium contents.

Research Products

• [Journal Article] Sodium chloride promotes tissue inflammation via osmotic stimuli in sub-total-nephrectomized mice. (2017)
  • Author(s): Sakata F., Ito Y., Mizuno M, Sawai A, Suzuki Y, Tomita T, Tawada M, Tanaka A, Hirayama A, Sagara A, Wada T, Maruyama S, Soga T, Matsuo S, Imai E, Takei Y.
  • Journal Title: Lab Invest Volume: 97(4) Issue: 4 Pages: 432-446
  • DOI: 10.1038/labinvest.2017.4
  • Peer Reviewed / Open Access
• [Journal Article] T cells bearing anti-CD19 and/or anti-CD38 chimeric antigen receptors effectively abrogate primary double-hit lymphoma cells (2017)
  • Author(s): Mihara K, Yoshida T, Takei Y, Sasaki N, Takihara Y, Kuroda J, Ichinohe T.
  • Journal Title: J Hematol Oncol Volume: 10 Issue: 1 Pages: 116-116
  • DOI: 10.1186/s13045-017-0488-x
  • Peer Reviewed
• [Journal Article] Apoptosis inhibitor of macrophage ameliorates fungus-induced peritoneal injury model in mice (2017)
  • Author(s): Tomita T, Arai S, Kitada K, Mizuno M, Suzuki Y, Sakata F, Nakano D, Hiramoto E, Takei Y, Maruyama S, Nishiyama A, Matsuo S, Miyazaki T, Ito Y.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 6450-6450
  • DOI: 10.1038/s41598-017-06824-6
  • Peer Reviewed / Open Access
• [Journal Article] The microRNA miR-516a-3p regulates the Wnt pathway by targeting extracellular sulfatase 1 in human scirrhous gastric cancers: Anti-metastatic therapy via miRNA-based medicine. (2017)
  • Author(s): Takei Y, Suzuki A, Mihara K, Yanagihara K.
  • Journal Title: Med Res Arch Volume: 5 Pages: 1-19
  • Peer Reviewed / Open Access
• [Journal Article] Vascular endothelial cell injury is an important factor in the development of encapsulating peritoneal sclerosis in long-term peritoneal dialysis patients. (2016)
  • Author(s): Tawada M, Ito Y, Hamada C, Honda K, Mizuno M, Suzuki Y, Sakata F, Terabayashi T, Matsukawa Y, Maruyama S, Imai E, Matsuo S, Takei Y.
  • Journal Title: PLoS One Volume: 11 Issue: 4 Pages: e0154644-e0154644
  • DOI: 10.1371/journal.pone.0154644
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A therapeutic trail of human melanoma with combined small interfering RNAs targeting adaptor molecules p130Cas and paxillin activated under expression of ganglioside GD3. (2016)
  • Author(s): Makino Y, Hamamura K, Takei Y, Bhuiyan RH, Ohkawa Y, Ohmi Y, Nakashima H, Furukawa K, Furukawa K.
  • Journal Title: Biochimica et Biophysica Acta Volume: in press Issue: 8 Pages: 1753-1763
  • DOI: 10.1016/j.bbagen.2016.04.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] All-trans retinoic acid and interferon-α increase CD38 expression on adult T-cell leukemia cells and sensitize them to T cells bearing anti-CD38 chimeric antigen receptors. (2016)
  • Author(s): Mihara K, Yoshida T, Ishida S, Takei Y, Kitanaka A, Shimoda K, Morishita K, Takihara Y, Ichinohe T.
  • Journal Title: Blood Cancer J Volume: 6 Issue: 5 Pages: e421-e421
  • DOI: 10.1038/bcj.2016.30
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] All-trans retinoic acid enhances cytotoxic effect of T cells with an anti-CD38 chimeric antigen receptor in acute myeloid leukemia. (2016)
  • Author(s): Yoshida T, Mihara K, Takei Y, Yanagihara K, Kubo T, Bhattacharyya J, Imai C, Mino T, Takihara Y, Ichinohe T.
  • Journal Title: Clin Trans Immunol Volume: 5 Issue: 12
  • DOI: 10.1038/cti.2016.73
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Atelocollagen-Mediated Intravenous siRNA Delivery Specific to Tumor Tissues Orthotopically Xenografted in Prostates of Nude Mice and Its Anticancer Effects. (2015)
  • Author(s): Yuan Y, Makita N, Cao D, Mihara K, Kadomatsu K, Takei Y.
  • Journal Title: Nucleic Acid Ther. Volume: 25 Issue: 2 Pages: 85-94
  • DOI: 10.1089/nat.2014.0526
  • Peer Reviewed
• [Journal Article] Vascular endothelial growth factor receptor-3 is a novel target to improve net ultrafiltration in methylglyoxal-induced peritoneal injury (2015)
  • Author(s): Terabayashi T, Ito Y, Mizuno M, Suzuki Y, Kinashi H, Sakata F, Tomita T, Iguchi D, Tawada M, Nishio R, Maruyama S, Imai E, Matsuo S, Takei Y.
  • Journal Title: Laboratory Investigation Volume: 95(9) Issue: 9 Pages: 1029-1043
  • DOI: 10.1038/labinvest.2015.87
  • Peer Reviewed
• [Presentation] スキルス胃癌患者由来のリンパ行性転移細胞株の樹立とその抗転移療法への応用 (2017)
  • Author(s): 武井佳史、原敏文、落谷孝広、柳原五吉
  • Organizer: 第７６回 日本癌学会学術総会
• [Presentation] ヒトスキルス胃がんの網羅的転写解析による新たな転移シグネチャーの抽出 (2017)
  • Author(s): 原敏文、柳原五吉、武井佳史
  • Organizer: 第７６回 日本癌学会学術総会
• [Presentation] スキルス胃癌の腹膜転移を制御する代謝酵素の解析 (2016)
  • Author(s): 鈴木亜希子、三原圭一朗、柳原五吉、武井佳史
  • Organizer: 第８９回日本生化学会大会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-09-25
• [Presentation] スキルス胃癌の代謝を調節するmicroRNAを新規標的とした抗腹膜転移療法 (2015)
  • Author(s): 武井佳史、鈴木亜希子、三原圭一朗、柳原五吉
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 上皮間葉変換を制御するmiRNAを標的としたスキルス胃癌の腹膜転移抑止 (2015)
  • Author(s): 武井佳史、柳原五吉
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08