| Project/Area Number |
15K15074
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Kiyono Tohru 国立研究開発法人国立がん研究センター, 研究所, 分野長 (10186356)
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| Research Collaborator |
Yugawa Takashi
Tomomi Nakahara
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 不死化 / ヒト幹細胞 / 再生医療 / 上皮幹細胞 / 再生 / がん幹細胞 / 異種動物 / がん化 |
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| Outline of Final Research Achievements |
Normal human epithelial cells from pancreas, bile duct, gall bladder, liver, alveoli, stomach were isolated and transduced with HPV16 E6E7, MYC and mutant RAS (EMR) which were regulated by tetOff system so that we obtained easily maintained stable cell lines. These cells except for liver cells formed tumors when transplanted subcutaneously in nude mice depending on EMR expression and the tumors started shrinking within a few days after doxycyclin administration. All the tumors pathologically showed undifferentiated adenocarcinoma. After the regression, remaining tissues showed simple columnar epithelia sometimes filled with mucin which histologically resemble glands of original organs. Thus we have succeeded to reconstruct human tissues resembling original organs in nude mice.
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