Research Project
Grant-in-Aid for Challenging Exploratory Research
Recent studies suggest that three dimensional structures of chromosomes have important roles in transcriptional regulation or chromosomal replication, however, the mechanisms underlying forming chromosomal three dimensional structures remains unknown. In this work, we made attempt to understand the function of the DNA sequence that we found in a distance of 500 kb from Meis2 promoter and interacting to Meis2 promoter regardless of the transcriptional activity of Meis2.We tried and succeeded to establish conditional knockout ES cells and mouse line in this funding period. We also found that this DNA region has impact to transcription activities of Meis2 promoter by the conditional deletion of this DNA region. This transcriptional pattern alterations are very complex and out of expectations, and thus the DNA region reveals that it is not simple enhancer sequence. And also, preliminary results suggest that entire knockout homozygotes are lethal in the very early stages (blastocyst?).
All 2016 2015
All Journal Article (6 results) (of which Int'l Joint Research: 2 results, Peer Reviewed: 6 results, Open Access: 4 results, Acknowledgement Compliant: 3 results) Presentation (5 results) (of which Invited: 3 results)
Genes Nutr.
Volume: 11 Issue: 1 Pages: 1-1
10.1186/s12263-016-0534-2
Mol. Nutr. Food Res.
Volume: 61 Issue: 2 Pages: 1-15
10.1002/mnfr.201600477
Development
Volume: 143 Pages: 276-285
10.1242/dev.127506
Trends Biochem. Sci.
Volume: 41 Issue: 1 Pages: 9-19
10.1016/j.tibs.2015.11.005
Cell
Volume: 163 Issue: 2 Pages: 367-380
10.1016/j.cell.2015.08.058
Biosci. Biotechnol. Biochem.
Volume: 18 Pages: 1-5
