| Project/Area Number |
15K15097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MIKI Yasuhiro 東北大学, 災害科学国際研究所, 講師 (50451521)
ONO Katsuhiko 東北大学, 大学院医学系研究科, 技術専門職員 (80466531)
ONODERA Yoshiaki 東北大学, 大学病院, 技術専門職員 (40466561)
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| Research Collaborator |
IWABUCHI Erina 東北大学, 大学院医学系研究科, 技術補佐員
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | HER2 / CEACAM6 / タンパク質間相互作用 / 乳癌 / タンパク質相互作用 |
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| Outline of Final Research Achievements |
It has become important to identify the surrogate markers regarding HER2 inhibitor sensitivity for HER2-positive breast cancer patients. In this study, we focused on protein-protein interaction (PPI) between HER2 and carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) in breast cancer cells. In breast cancer RT-474 cells, HER2/CEACAM6 PPI was detected by using co-immunoprecipitation assay and visualized by using both proximity ligation assay (PLA) and high resolution microscopy. We then visualized HER2/CEACAM6 PPI by using PLA in human breast carcinoma tissues. Knockdown of CEACAM6 gene significantly decreased the anti-cell proliferative effect of HER2 inhibitor in RT-474. These findings suggest that CEACAM6 has an effect by PPI with HER2 on HER2 sensitivity in breast cancer cells.
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