Quantitative evaluation of estrogen receptor and the validation for breast cancer by nano particle-based immunohistochemistry

• Project/Area Number: 15K15108
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Tohoku University
• Principal Investigator: Yoshida Noriko 東北大学, 医学系研究科, 大学院非常勤講師 (70749788)
• Co-Investigator(Kenkyū-buntansha): 石田 孝宣 東北大学, 医学系研究科, 准教授 (00292318) ; 多田 寛 東北大学, 大学病院, 講師 (50436127) ; 宮下 穣 東北大学, 大学病院, 助教 (60710788) ; 渡部 剛 東北大学, 医学系研究科, 助教 (70451573) ; 大内 憲明 東北大学, 医学系研究科, 教授 (90203710)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 乳癌 / ER / 蛍光ナノ粒子 / エストロゲン受容体 / プロゲステロン受容体

Outline of Final Research Achievements

We have developed highly quantitative method to evaluate expression of ER and PgR in vitro. The expressing diverse level of ER and PgR in breast cancer specimens are stained by nano particle-based IHC. ER positive breast cancer cell lines (MCF7, T47D, BT474), ER negative breast cancer cell lines (ZR-75-1, MDA-MB-231) are processed into paraffin-embedded cell blocks. Then samples are separately stained by IHC with DAB (DAB-IHC) and nano particles (nano-IHC). Results of flow cytometry showed correlation to that of nano-IHC. The results of nano-IHC score showed that fluorescence of ER positive cells was increased in an expression level dependent manner which was difficultly distinguished by DAB-IHC. This study indicated that in breast cancer specimens, nano-IHC is generate sensitively quantitative than DAB-IHC. Thus nano-IHC might be provide highly quantitative method to evaluate the expression of ER and PgR, which were proposed in the new IHC-based surrogate biomarker for breast cancer.

Research Products

• [Journal Article] Predictive diagnosis of the risk of breast cancer recurrence after surgery by single-particle quantum dot imaging. (2015)
  • Author(s): Gonda K, Miyashita M, Higuchi H, Tada H, Watanabe TM, Watanabe M, Ishida T, Ohuchi N.
  • Journal Title: Scientific Report. Volume: 22(5) Issue: 1 Pages: 14322-14322
  • DOI: 10.1038/srep14322
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Highly Sensitive Imaging of Cancer with Functional Nanoparticles. (2015)
  • Author(s): Gonda K, Hamada Y, Kitamura N, Tada H, Miyashita M, Kamei T, Ishida T, Ohuchi N.
  • Journal Title: Journal of Photopolymer Science and Technology. Volume: 28 Pages: 701-706
  • NAID: 130005108630
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clinical significance of subtype classification in metastatic lymph nodes of breast cancer patients undergoing neoadjuvant chemotherapy. (2015)
  • Author(s): Nemoto N1, Shibahara Y, Tada H, Uchida K, McNamara KM, Chan MS, Watanabe M, Tamaki K, Miyashita M, Miki Y, Gonda K, Ishida T, Ohuchi N, Sasano H.
  • Journal Title: Int J Biol Markers. Volume: in press Issue: 2 Pages: 174-183
  • DOI: 10.5301/jbm.5000128
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Quantitative evaluation methods of hormone receptors in / vitro and breast cancer patient specimens /by new fluorescence nanoparticle-based immunohistochemistry(IHC) (2017)
  • Author(s): Zhaorong Guo, Hiroshi Tada, Narufumi kitamura, Yoh Hamada, Kohsuke Gonda, Noriaki Ohuchi
  • Organizer: A3 symposium
  • Place of Presentation: 韓国、ソウル
  • Year and Date: 2017-01-16
  • Int'l Joint Research
• [Presentation] Highly quantitative evaluation methods of breast cancer biomarker using novel fluorescence nano-particles (2016)
  • Author(s): Zhaorong Guo, Hiroshi Tada, Narufumi kitamura, Yoh Hamada, Kohsuke Gonda, Noriaki Ohuchi
  • Organizer: 第75回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜市, 神奈川県)
  • Year and Date: 2016-10-07
• [Presentation] Highly quantitative evaluation methods of progesterone receptor for breast cancer subtype classification by immunohistochemistry using fluorescence nano-particles (2016)
  • Author(s): Zhaorong Guo, Hiroshi Tada, Narufumi kitamura, Yoh Hamada, Kohsuke Gonda, Noriaki Ohuchi
  • Organizer: ナノ学会 第14回大会
  • Place of Presentation: 北九州国際会議場(福岡県, 北九州市)
  • Year and Date: 2016-06-16
• [Presentation] New diagnostic method of HER2 in breast cancer using single fluorescence nanoparticle imaging (2015)
  • Author(s): 宮下 穣
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋・名古屋国際会議場
  • Year and Date: 2015-05-01