Three-dimensional culture of reprogrammed brown adipocytes

Research Project

Project/Area Number	15K15111
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Experimental pathology
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Mazda Osam 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00271164)
Co-Investigator(Renkei-kenkyūsha)	Kawahito Yutaka 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (50336731) Gojo Satoshi 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745) Kishida Tsunao 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205)
Project Period (FY)	2015-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords	糖尿病 / 褐色脂肪細胞
Outline of Final Research Achievements	We established a procedure to directly convert fibroblasts into brown adipocytes. We also found that transplantation of the brown adipocytes remarkably suppressed diabetes in mice. Three-dimensional culture of the brown adipocytes may further improve the viability and metabolic activity of the cells in vivo after transplantation. Then we aimed at forming 3D brown adipose tissue using the reprogrammed brown adipocytes. After various trials, we observed some characteristic structure by co-culturing different lineages of the cells. The present findings may implicate a novel tissue engineering procedure that may be useful for regenerative therapy for diabetes mellitus.