| Project/Area Number |
15K15113
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Kindai University |
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Principal Investigator |
ITO Akihiko 近畿大学, 医学部, 教授 (80273647)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 内圧上昇 / 変性 / 緑内障 / 接着分子 / メカノバイオロジー / メカノパソロジー / two-chamber培養装置 / 病的内圧上昇 / 組織変性 / 細胞変性 / アポトーシス |
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| Outline of Final Research Achievements |
Elevation of intraluminal, such as intraocular and intra-renal pelvic, pressure causes neuronal and mucosal degeneration. The threshold is fairly low and constant, approximately 30 cmH2O. A new two-chamber culture system was devised wherein cells were cultured on a semipermeable membrane and subjected to increased medium height (water pressure up to 60 cmH2O). Primary cultured mouse neurons were found to fall into axonal degeneration when loaded with 30 cmH2O or more, in association with increased ectodomain shedding of CADM1, an IgCAM member expressed on neuritic cell membrane. The cell-side remnant produced by shedding appeared to be involved in axonal degeneration. Columnar epithelial cells were flattened and growth suppressed when loaded with 30 cmH2O or more. This work contributes to our understanding the pathophysiology of the development of degenerative lesions caused by low static pressure burden.
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