| Project/Area Number |
15K15114
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | National Defense Medical College |
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Principal Investigator |
MATSUMURA Kouji 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 医学教育部共同利用研究施設, 講師 (30272610)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAHIRA Yasushi 防衛医科大学校, 医学教育部, 教授 (40265781)
ISHIHARA Miya 防衛医科大学校, 医学教育部, 教授 (30505342)
MIZUNO Hiroshi 順天堂大学, 医学部, 教授 (80343606)
ISHIHARA Masayuki 防衛医学研究センター, 教授 (10508500)
OKADA Yoshikiyo 防衛医科大学校, 医学教育部, 助教 (90531137)
ARAI Masaaki 防衛医科大学校, 医学教育部, 講師 (50534864)
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| Research Collaborator |
市来 やよい 防衛医科大学校, 医学教育部
梅山 悠伊 順天堂大学, 医学部
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | iPS細胞 / 奇形腫 / ソーティング / 細胞培養 / 分化 / 血管内皮細胞 / 線維芽細胞 / 神経細胞 / ヒトiPS細胞 / KRT5 / OLFM4 / LGR5 / CFTR / CPM / 多能性幹細胞 / 分化誘導 / 気道上皮 / 細胞分散 |
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| Outline of Final Research Achievements |
A new induction method using teratoma was developed. The method is transplanting human iPS cells (hiPSC) to SCID mice, sorting from teratoma cells with surface markers, and culturing. When CD34 positive cells were cultured in vascular endothelial cell medium, CD31, CD144 positive vascular endothelial cells appeared. Moreover, expression of fibroblast antigen was observed in anti-fibroblast antibody positive cells. CD56 (NCAM) positive cells after sorting were neural progenitor-like cells, and differentiated cells including neurites were observed. Thus, differentiation-induced cells could be removed by sorting hiPSC teratoma cells with cell markers. This in vivo differentiation induction method was thought to make it possible to isolate differentiated cells that are difficult to induce.
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