| Project/Area Number |
15K15129
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Mimuro Hitomi 東京大学, 医科学研究所, 准教授 (80396887)
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| Co-Investigator(Kenkyū-buntansha) |
氣駕 恒太朗 自治医科大学, 医学部, 講師 (90738246)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細菌 / 感染症 / sRNA |
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| Outline of Final Research Achievements |
To discover novel effector RNAs of Helicobacer pylori, we analyzed H. pylori-coding small RNAs (sRNAs) and identified RNAs having potential to be translocated from bacteria to host cells. Comprehensive RNA-seq data of H. pylori were generated by next generation sequencers, and the expression levels of sRNA were determined by quantitative PCR to identify sRNA candidates. Moreover, some of the sRNAs were specifically condensed in bacterial outer membrane vesicles, indicating that the particular sRNAs possess capacity to be translocated from H. pylori into the host cells.
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